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Transcriptional patterns of growth factors and
proto-oncogenes in human glioblastomas and normal glial cells
Gillaspy GE, Mapstone TB, Samols D, Goldthwait DA
Department of Biochemistry, Case Western Reserve University,
Cleveland, Ohio 44106
To document over-expression of proto-oncogenes in tumors, it is necessary to
determine the level of expression in the progenitor normal tissue.
These studies compare the levels of nuclear transcription of a series of
growth-factor related genes and proto-oncogenes in human glioblastoma cell lines
with those in three normal glial cell populations.
The unusual finding was that levels in the three normal glial cell populations
varied considerably for several genes and thus overexpression of a specific gene
in a tumor cell when compared to just one normal glial cell population would not
necessarily represent overexpression.
In this study, we compared the level of 17 genes in 7 tumors to the highest
level of each gene found in any of three normal glial cell populations.
Over-expression of PDGF-B in 4/7 glioblastoma cell lines, EGFR in 1/7, neu in
1/7 IGF-2 in 1/7 and ros in 2/7 was observed.
The variation observed in the normal glial cell populations emphasizes the
possibility that the normal glial cell populations represent different glial
cell lineages and/or stages of differentiation and that the tumors could have
arisen from different normal glial cells.
Matching lineages of normal and tumor cells, probably by monoclonal antibody
reactions, may be required to accurately define over-expression.
PMID: 1324785 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1324785&dopt=Abstract
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