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Characterisation
of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary
hormones
Savarese A, Annicchiarico-Petruzzelli M, Citro G, Zupi G, Spagnoli LG,
Colantoni A, Vernole P, Stephanou A, Knight RA, Guerrieri P, et al
Universita Rome Tor Vergata, Dip. Medicina Sperimentale, Italy.
The human glioblastoma cell
line LI showed morphological features typical of its neuroectodermal
origin.
Cells were positive by immunofluorescence to GFAP, MHC class II, and L1
determinants.
Cytogenetic analysis showed the presence of a modal chromosome number of 63,
ranging from 58 to 69 chromosomes (DNA index was 1.6).
Northern blot analysis demonstrated the presence of mRNA transcripts specific
for transglutaminase C (type II or "tissue"), growth-hormone
releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and
proopiomelanocortin (POMC).
The GHRH mRNA was present in two different sizes, one similar to the normal
hypothalamic species of 0.75 kb, whilst the second species was a large
transcript of approximately 10 kb size.
Treatment with 5 microM retinoic acid or 5 mM alpha-difluoromethylornithine for
5 days sharply reduced the growth rate and also induced modulation of the
ultrastructure and antigenic profile.
This cell line may be useful to study glial differentiation and the relationship
of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal
tumours.
PMID: 1623982 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1623982&dopt=Abstract
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