Etiology and Pathogenesis > Insulin-like Growth Factors  

Abstract

Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA

Trojan J, Johnson TR, Rudin SD, Ilan J, Tykocinski ML, Ilan J

INSERM, Paris

Rat C6 glioma cells express insulin-like growth factor I (IGF-I) and form rapidly growing tumors in syngeneic animals. 
When transfected with an episome-based vector encoding antisense IGF-I complementary DNA, these cells lost tumorigenicity. 
Subcutaneous injection of IGF-I antisense-transfected C6 cells into rats prevented formation of both subcutaneous tumors and brain tumors induced by nontransfected C6 cells. 
The antisense-transfected cells also caused regression of established brain glioblastomas when injected at a point distal to the tumor. 
These antitumor effects result from a glioma-specific immune response involving CD8+ lymphocytes. 
Antisense blocking of IGF-I expression may reverse a phenotype that allows C6 glioma cells to evade the immune system.

PMID: 8418502 [PubMed - indexed for MEDLINE]