From: Subject: Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor -- Gritti et al. 16 (3): 1091 -- Journal of Neuroscience Date: Sun, 20 Jan 2008 14:43:15 +0100 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0000_01C85B72.C9DBE550" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0000_01C85B72.C9DBE550 Content-Type: text/html; charset="Windows-1252" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.jneurosci.org/cgi/content/abstract/16/3/1091?ijkey=47d01867a2f4e4df3502a4d3e3a0410359ef84c6&keytype2=tf_ipsecsha Multipotential stem cells from the adult mouse brain = proliferate and self-renew in response to basic fibroblast growth factor = -- Gritti et al. 16 (3): 1091 -- Journal of Neuroscience
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Journal of Neuroscience, Vol 16, 1091-1100, Copyright = =A9 1996 by=20 Society for Neuroscience

ARTICLE

Multipotential stem cells from the adult mouse brain proliferate and = self-renew in response to basic fibroblast growth factor

A Gritti, EA Parati, L Cova, P Frolichsthal, R Galli, E = Wanke, L=20 Faravelli, DJ Morassutti, F Roisen, DD Nickel and AL Vescovi =
Laboratory of Cellular Neuropharmacology, National = Neurological=20 Institute C, Milan, Italy.=20

It has been established that the adult mouse forebrain contains=20 multipotential (neuronal/glial) progenitor cells that can be = induced=20 to proliferate in vitro when epidermal growth factor is = provided.=20 These cells are found within the subventricular zone of the = lateral=20 ventricles, together with other progenitor cell populations, = whose=20 requirements for proliferation remain undefined. Using basic=20 fibroblast growth factor (bFGF), we have isolated = multipotential=20 progenitors from adult mouse striatum. These progenitors = proliferate=20 and can differentiate into cells displaying the antigenic = properties=20 of astrocytes, oligodendrocytes, and neurons. The neuron-like = cells=20 possess neuronal features, exhibit neuronal = electrophysiological=20 properties, and are immunoreactive for GABA, substance P, = choline=20 acetyl-transferase, and glutamate. Clonal analysis confirmed the=20 multipotency of these bFGF-dependent cells. Most significantly,=20 subcloning experiments demonstrated that they were capable of = self-=20 renewal, which led to a progressive increase in population = size over=20 serial passaging. These results demonstrate that bFGF is = mitogenic=20 for multipotential cells from adult mammalian forebrain that = possess=20 stem cell properties.

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Generation of Functional Radial = Glial Cells=20 by Embryonic and Adult Forebrain Neural Stem Cells
J.=20 Neurosci., December 17, 2003; 23(37): 11587 - 11601. =
[Abstr= act]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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H.=20 Li, G. Roblin, H. Liu, and S. Heller
From The Cover: = Generation=20 of hair cells by stepwise differentiation of embryonic stem=20 cells
PNAS, November 11, 2003; 100(23): = 13495 -=20 13500.
[Abstract]= =20 [Full=20 Text] [PDF]=20
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H.=20 Toda, M. Tsuji, I. Nakano, K. Kobuke, T. Hayashi, H. Kasahara, J.=20 Takahashi, A. Mizoguchi, T. Houtani, T. Sugimoto, et=20 al.
Stem Cell-derived Neural Stem/Progenitor Cell=20 Supporting Factor Is an Autocrine/Paracrine Survival Factor for = Adult=20 Neural Stem/Progenitor Cells
J. Biol. Chem.,=20 September 12, 2003; 278(37): 35491 - 35500.
[Abstract]<= /A>=20 [Full = Text]=20 [PDF] =
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R.=20 Cancedda, G. Bianchi, A. Derubeis, and R. Quarto
Cell = Therapy=20 for Bone Disease: A Review of Current Status
Stem = Cells,=20 September 1, 2003; 21(5): 610 - 619.
[Abstract]=20 [Fu= ll=20 Text] [PDF]=20
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M. S.=20 Ridyard and S. M. Robbins
Fibroblast Growth = Factor-2-induced=20 Signaling through Lipid Raft-associated Fibroblast Growth Factor = Receptor=20 Substrate 2 (FRS2)
J. Biol. Chem., = April 11, 2003;=20 278(16): 13803 - 13809.
[Abstract]<= /A>=20 [Full = Text]=20 [PDF] =
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R.=20 Galli, A. Gritti, L. Bonfanti, and A. L. Vescovi
Neural = Stem=20 Cells: An Overview
Circ. Res., = April 4, 2003; 92(6):=20 598 - 608.
[Ab= stract]=20 [Full=20 Text] [PDF]=20
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R.=20 Dono
Fibroblast growth factors as regulators of central = nervous=20 system development and function
Am J Physiol = Regulatory=20 Integrative Comp Physiol, April 1, 2003; 284(4): R867 - = R881.=20
[A= bstract]=20 [Full = Text] [PDF]=20
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M.=20 Ishibashi and A. P. McMahon
A sonic hedgehog-dependent=20 signaling relay regulates growth of diencephalic and mesencephalic = primordia in the early mouse embryo
Development,=20 March 12, 2003; 129(20): 4807 - 4819.
[Abst= ract]=20 [Full=20 Text] [PDF]=20
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C.=20 Vicario-Abejon, M. J. Yusta-Boyo, C. Fernandez-Moreno, and F. de=20 Pablo
Locally Born Olfactory Bulb Stem Cells = Proliferate in=20 Response to Insulin-Related Factors and Require Endogenous = Insulin-Like=20 Growth Factor-I for Differentiation into Neurons and = Glia
J.=20 Neurosci., February 1, 2003; 23(3): 895 - 906.
[Abstract= ]=20 [Full=20 Text] [PDF] =
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M.=20 V.T. Lobo, F. J. M. Alonso, C. Redondo, M. A. Lopez-Toledano, E. = Caso, A.=20 S. Herranz, C. L. Paino, D. Reimers, and E. = Bazan
Cellular=20 Characterization of Epidermal Growth Factor-expanded Free-floating = Neurospheres
J. Histochem. Cytochem.,=20 January 1, 2003; 51(1): 89 - 103.
[Abstract] = [Full = Text] [PDF]
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G. Q.=20 Daley, M. A. Goodell, and E. Y. Snyder
Realistic = Prospects for=20 Stem Cell Therapeutics
Hematology, = January 1, 2003;=20 2003(1): 398 - 418.
[Abstract]=20 [Full=20 Text] [PDF]=20
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N.=20 Picard-Riera, L. Decker, C. Delarasse, K. Goude, B. Nait-Oumesmar, = R.=20 Liblau, D. Pham-Dinh, and A. B.-V. = Evercooren
Experimental=20 autoimmune encephalomyelitis mobilizes neural progenitors from the = subventricular zone to undergo oligodendrogenesis in adult=20 mice
PNAS, October 1, 2002; 99(20): 13211 - = 13216.=20
[Abstract]<= /A>=20 [Full = Text]=20 [PDF] =
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J. M.=20 Parent, V. V. Valentin, and D. H. Lowenstein
Prolonged = Seizures=20 Increase Proliferating Neuroblasts in the Adult Rat Subventricular = Zone-Olfactory Bulb Pathway
J. Neurosci.,=20 April 15, 2002; 22(8): 3174 - 3188.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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R. M.=20 Seaberg and D. van der Kooy
Adult Rodent Neurogenic = Regions:=20 The Ventricular Subependyma Contains Neural Stem Cells, But the = Dentate=20 Gyrus Contains Restricted Progenitors
J. Neurosci.,=20 March 1, 2002; 22(5): 1784 - 1793.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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J.=20 Evans, C. Sumners, J. Moore, M. J. Huentelman, J. Deng, C. H. = Gelband, and=20 G. Shaw
Characterization of Mitotic Neurons Derived = From Adult=20 Rat Hypothalamus and Brain Stem
J Neurophysiol,=20 February 1, 2002; 87(2): 1076 - 1085.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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A.=20 Gritti, L. Bonfanti, F. Doetsch, I. Caille, A. Alvarez-Buylla, D. = A. Lim,=20 R. Galli, J. M. G. Verdugo, D. G. Herrera, and A. L.=20 Vescovi
Multipotent Neural Stem Cells Reside into the = Rostral=20 Extension and Olfactory Bulb of Adult Rodents
J. = Neurosci.,=20 January 15, 2002; 22(2): 437 - 445.
[Abstract= ]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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R.=20 Galli, R. Fiocco, L. De Filippis, L. Muzio, A. Gritti, S. = Mercurio, V.=20 Broccoli, M. Pellegrini, A. Mallamaci, and A. L. = Vescovi
Emx2=20 regulates the proliferation of stem cells of the adult mammalian = central=20 nervous system
Development, January 4, 2002; = 129(7):=20 1633 - 1644.
[Abstr= act]=20 [Full=20 Text] [PDF] =
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S.=20 Eridani
Stem cells for all seasons? Experimental and = clinical=20 issues
J. R. Soc. Med., January 1, 2002; = 95(1): 5 -=20 8.
[Full=20 Text] [PDF]=20
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S.-i.=20 Yamamoto, M. Nagao, M. Sugimori, H. Kosako, H. Nakatomi, N. = Yamamoto, H.=20 Takebayashi, Y.-i. Nabeshima, T. Kitamura, G. Weinmaster, et=20 al.
Transcription Factor Expression and = Notch-Dependent=20 Regulation of Neural Progenitors in the Adult Rat Spinal=20 Cord
J. Neurosci., December 15, 2001; = 21(24): 9814 -=20 9823.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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A. G.=20 Trentin, M. Alvarez-Silva, and V. Moura Neto
Thyroid = hormone=20 induces cerebellar astrocytes and C6 glioma cells to secrete = mitogenic=20 growth factors
Am J Physiol Endocrinol Metab,=20 November 1, 2001; 281(5): E1088 - E1094.
[= Abstract]=20 [Full= =20 Text] [PDF] =
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C.-C.=20 Shih, Y. Weng, A. Mamelak, T. LeBon, M. C.-T. Hu, and S. J.=20 Forman
Identification of a candidate human = neurohematopoietic=20 stem-cell population
Blood, = October 15, 2001; 98(8):=20 2412 - 2422.
[Abstract]=20 [Full=20 Text] = [PDF]=20
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C. H.=20 Faux, A. M. Turnley, R. Epa, R. Cappai, and P. F.=20 Bartlett
Interactions between Fibroblast Growth Factors = and=20 Notch Regulate Neuronal Differentiation
J. Neurosci.,=20 August 1, 2001; 21(15): 5587 - 5596.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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V. T.=20 Chu and F. H. Gage
Chipping away at stem=20 cells
PNAS, July 3, 2001; 98(14): 7652 - = 7653.=20
[Full=20 Text] [PDF]=20
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L. S.=20 Shihabuddin, P. J. Horner, J. Ray, and F. H. Gage
Adult = Spinal=20 Cord Stem Cells Generate Neurons after Transplantation in the = Adult=20 Dentate Gyrus
J. Neurosci., = December 1, 2000;=20 20(23): 8727 - 8735.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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S. F.=20 Pagano, F. Impagnatiello, M. Girelli, L. Cova, E. Grioni, M. = Onofri, M.=20 Cavallaro, S. Etteri, F. Vitello, S. Giombini, et=20 al.
Isolation and Characterization of Neural Stem = Cells=20 from the Adult Human Olfactory Bulb
Stem Cells,=20 July 1, 2000; 18(4): 295 - 300.
[Abstract]=20 [Fu= ll=20 Text]
3D""=20 = 		3D""=20
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M.-C.=20 Amoureux, B. A. Cunningham, G. M. Edelman, and K. L.=20 Crossin
N-CAM Binding Inhibits the Proliferation of = Hippocampal=20 Progenitor Cells and Promotes Their Differentiation to a Neuronal=20 Phenotype
J. Neurosci., May 15, 2000; = 20(10): 3631 -=20 3640.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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F. H.=20 Gage
Mammalian Neural Stem Cells
Science,=20 February 25, 2000; 287(5457): 1433 - 1438.
[Ab= stract]=20 [Full=20 Text]
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D. J.=20 Martens, V. Tropepe, and D. van der Kooy
Separate = Proliferation=20 Kinetics of Fibroblast Growth Factor-Responsive and Epidermal = Growth=20 Factor-Responsive Neural Stem Cells within the Embryonic Forebrain = Germinal Zone
J. Neurosci., = February 1, 2000; 20(3):=20 1085 - 1095.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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M.=20 Zappone, R Galli, R Catena, N Meani, S De Biasi, E Mattei, C = Tiveron, A.=20 Vescovi, R Lovell-Badge, S Ottolenghi, et = al.
Sox2=20 regulatory sequences direct expression of a (beta)-geo transgene = to=20 telencephalic neural stem cells and precursors of the mouse = embryo,=20 revealing regionalization of gene expression in CNS stem=20 cells
Development, January 6, 2000; 127(11): = 2367 -=20 2382.
[Abst= ract]=20 [PDF]=20
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J Xu,=20 Z Liu, and D. Ornitz
Temporal and spatial gradients of = Fgf8 and=20 Fgf17 regulate proliferation and differentiation of midline = cerebellar=20 structures
Development, January 5, 2000; = 127(9):=20 1833 - 1843.
[Abstr= act]=20 [PDF]=20
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H.=20 Harada, P. Kettunen, H.-S. Jung, T. Mustonen, Y. A. Wang, and I.=20 Thesleff
Localization of Putative Stem Cells in Dental=20 Epithelium and Their Association with Notch and FGF=20 Signaling
J. Cell Biol., October 4, 1999; = 147(1):=20 105 - 120.
[Abstract]= [Full = Text] [PDF] =
=20 		3D""=20
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G. C.=20 Kopen, D. J. Prockop, and D. G. Phinney
Marrow stromal = cells=20 migrate throughout forebrain and cerebellum, and they = differentiate into=20 astrocytes after injection into neonatal mouse = brains
PNAS,=20 September 14, 1999; 96(19): 10711 - 10716.
[Abstract]<= /A>=20 [Full = Text]=20 [PDF] =
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P. C.=20 Mabie, M. F. Mehler, and J. A. Kessler
Multiple Roles = of Bone=20 Morphogenetic Protein Signaling in the Regulation of Cortical Cell = Number=20 and Phenotype
J. Neurosci., August 15, 1999; = 19(16):=20 7077 - 7088.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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J. P.=20 Wagner, I. B. Black, and E. DiCicco-Bloom
Stimulation = of=20 Neonatal and Adult Brain Neurogenesis by Subcutaneous Injection of = Basic=20 Fibroblast Growth Factor
J. Neurosci.,=20 July 15, 1999; 19(14): 6006 - 6016.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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C. E.=20 Johanson, J. Szmydynger-Chodobska, A. Chodobski, A. Baird, P. = McMillan,=20 and E. G. Stopa
Altered formation and bulk absorption = of=20 cerebrospinal fluid in FGF-2-induced hydrocephalus
Am = J=20 Physiol Regulatory Integrative Comp Physiol, = July 1, 1999;=20 277(1): R263 - R271.
[A= bstract]=20 [Full = Text] [PDF]=20
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D. A.=20 Lim and A. Alvarez-Buylla
Interaction between = astrocytes and=20 adult subventricular zone precursors stimulates=20 neurogenesis
PNAS, June 22, 1999; 96(13): = 7526 -=20 7531.
[Abstract]=20 [Full = Text]=20 [PDF] =
=20 		3D""=20
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B. D.=20 Yandava, L. L. Billinghurst, and E. Y. Snyder
"Global" = cell=20 replacement is feasible via neural stem cell transplantation: = Evidence=20 from the dysmyelinated shiverer mouse brain
PNAS,=20 June 8, 1999; 96(12): 7029 - 7034.
[Abstract]=20 [Full = Text]=20 [PDF] =
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B. J.=20 Chiasson, V. Tropepe, C. M. Morshead, and D. van der = Kooy
Adult=20 Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate=20 Proliferative Potential, but only Subependymal Cells Have Neural = Stem Cell=20 Characteristics
J. Neurosci., June 1, 1999; = 19(11):=20 4462 - 4471.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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M. M.=20 Daadi and S. Weiss
Generation of Tyrosine = Hydroxylase-Producing=20 Neurons from Precursors of the Embryonic and Adult=20 Forebrain
J. Neurosci., June 1, 1999; = 19(11): 4484 -=20 4497.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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A.=20 Gritti, P. Frolichsthal-Schoeller, R. Galli, E. A. Parati, L. = Cova, S. F.=20 Pagano, C. R. Bjornson, and A. L. Vescovi
Epidermal and = Fibroblast Growth Factors Behave as Mitogenic Regulators for a = Single=20 Multipotent Stem Cell-Like Population from the Subventricular = Region of=20 the Adult Mouse Forebrain
J. Neurosci., = May 1, 1999;=20 19(9): 3287 - 3297.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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B.=20 Kirschenbaum, F. Doetsch, C. Lois, and A. = Alvarez-Buylla
Adult=20 Subventricular Zone Neuronal Precursors Continue to Proliferate = and=20 Migrate in the Absence of the Olfactory Bulb
J. = Neurosci.,=20 March 15, 1999; 19(6): 2171 - 2180.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
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C. R.=20 Bjornson, R. L. Rietze, B. A. Reynolds, M. C. Magli, and A. L.=20 Vescovi
Turning Brain into Blood: A Hematopoietic Fate = Adopted=20 by Adult Neural Stem Cells in Vivo
Science,=20 January 22, 1999; 283(5401): 534 - 537.
[Abs= tract]=20 [Full=20 Text]
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K=20 Sakurada, M Ohshima-Sakurada, T. Palmer, and F. = Gage
Nurr1, an=20 orphan nuclear receptor, is a transcriptional activator of = endogenous=20 tyrosine hydroxylase in neural progenitor cells derived from the = adult=20 brain
Development, January 9, 1999; 126(18): = 4017 -=20 4026.
[Abst= ract]=20 [PDF]=20
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E. Y.=20 Snyder
Review : Neural Stem-Like Cells: Developmental = Lessons=20 with Therapeutic Potential
Neuroscientist,=20 November 1, 1998; 4(6): 408 - 425.
[Abstract]=20 [PDF] =
=20 		3D""=20
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W.=20 Li, C. A. Cogswell, and J. J. LoTurco
Neuronal = Differentiation=20 of Precursors in the Neocortical Ventricular Zone Is Triggered by=20 BMP
J. Neurosci., November 1, 1998; 18(21): = 8853 -=20 8862.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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F.=20 Ciccolini and C. N. Svendsen
Fibroblast Growth Factor=20 2 (FGF-2) Promotes Acquisition of Epidermal Growth Factor = (EGF)=20 Responsiveness in Mouse Striatal Precursor Cells: Identification = of Neural=20 Precursors Responding to both EGF and FGF-2
J. = Neurosci.,=20 October 1, 1998; 18(19): 7869 - 7880.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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T.=20 Ben-Hur, B. Rogister, K. Murray, G. Rougon, and M.=20 Dubois-Dalcq
Growth and Fate of PSA-NCAM+ Precursors of = the=20 Postnatal Brain
J. Neurosci., = August 1, 1998;=20 18(15): 5777 - 5788.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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L.=20 Calza, L. Giardino, M. Pozza, C. Bettelli, A. Micera, and L.=20 Aloe
Proliferation and phenotype regulation in the=20 subventricular zone during experimental allergic = encephalomyelitis: In=20 vivo evidence of a role for nerve = growth factor
PNAS,=20 March 17, 1998; 95(6): 3209 - 3214.
[Abstract]=20 [Full = Text]=20 [PDF] =
=20 		3D""=20
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Y.=20 Arsenijevic and S. Weiss
Insulin-Like Growth Factor-I = Is a=20 Differentiation Factor for Postmitotic CNS Stem Cell-Derived = Neuronal=20 Precursors: Distinct Actions from Those of Brain-Derived = Neurotrophic=20 Factor
J. Neurosci., March 15, 1998; 18(6): = 2118 -=20 2128.
[Abstrac= t]=20 [Full=20 Text] [PDF] =
=20 		3D""=20
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R=20 Josephson, T Muller, J Pickel, S Okabe, K Reynolds, P. Turner, A = Zimmer,=20 and R. McKay
POU transcription factors control = expression of=20 CNS stem cell-specific genes
Development,=20 January 8, 1998; 125(16): 3087 - 3100.
[Abst= ract]=20 [PDF]=20
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C.=20 Morshead, C. Craig, and D van der Kooy
In vivo clonal = analyses=20 reveal the properties of endogenous neural stem cell proliferation = in the=20 adult mammalian forebrain
Development,=20 January 6, 1998; 125(12): 2251 - 2261.
[Abst= ract]=20 [PDF]=20
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S.-i.=20 Sakakibara and H. Okano
Expression of Neural = RNA-Binding=20 Proteins in the Postnatal CNS: Implications of Their Roles in = Neuronal and=20 Glial Cell Development
J. Neurosci.,=20 November 1, 1997; 17(21): 8300 - 8312.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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V.=20 Tropepe, C. G. Craig, C. M. Morshead, and D. van der=20 Kooy
Transforming Growth Factor-alpha Null and = Senescent Mice=20 Show Decreased Neural Progenitor Cell Proliferation in the = Forebrain=20 Subependyma
J. Neurosci., October 15, 1997; = 17(20):=20 7850 - 7859.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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H. G.=20 Kuhn, J. Winkler, G. Kempermann, L. J. Thal, and F. H.=20 Gage
Epidermal Growth Factor and Fibroblast Growth = Factor-2=20 Have Different Effects on Neural Progenitors in the Adult Rat=20 Brain
J. Neurosci., August 1, 1997; 17(15): = 5820 -=20 5829.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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F.=20 Doetsch, J. M. Garcia-Verdugo, and A. = Alvarez-Buylla
Cellular=20 Composition and Three-Dimensional Organization of the = Subventricular=20 Germinal Zone in the Adult Mammalian Brain
J. = Neurosci.,=20 July 1, 1997; 17(13): 5046 - 5061.
[Abstra= ct]=20 [Full=20 Text] [PDF] =
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J.=20 Ray, A. Baird, and F. H. Gage
A 10-amino acid sequence = of=20 fibroblast growth factor 2 is sufficient for its mitogenic = activity=20 on neural progenitor cells
PNAS, = June 24, 1997;=20 94(13): 7047 - 7052.
[Abstract]=20 [Full = Text]=20 [PDF] =
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R.=20 McKay
Stem Cells in the Central Nervous=20 System
Science, April 4, 1997; 276(5309): 66 = - 71.=20
[Abst= ract]=20 [Full=20 Text]
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Opera = requires=0A= the Sun Java Runtime Environment .=0A= =0A= by Andrew Gregory=0A= http://www.scss.com.au/family/andrew/webdesign/xmlhttprequest/=0A= =0A= This work is licensed under the Creative Commons Attribution License. To = view a=0A= copy of this license, visit = http://creativecommons.org/licenses/by-sa/2.5/ or=0A= send a letter to Creative Commons, 559 Nathan Abbott Way, Stanford, = California=0A= 94305, USA.=0A= =0A= Attribution: Leave my name and web address in this script intact.=0A= =0A= Not Supported in Opera=0A= ----------------------=0A= * user/password authentication=0A= * responseXML data member=0A= =0A= Not Fully Supported in Opera=0A= ----------------------------=0A= * async requests=0A= * abort()=0A= * getAllResponseHeaders(), getAllResponseHeader(header)=0A= =0A= */=0A= // IE support=0A= if (window.ActiveXObject && !window.XMLHttpRequest) {=0A= window.XMLHttpRequest =3D function() {=0A= var msxmls =3D new Array(=0A= 'Msxml2.XMLHTTP.5.0',=0A= 'Msxml2.XMLHTTP.4.0',=0A= 'Msxml2.XMLHTTP.3.0',=0A= 'Msxml2.XMLHTTP',=0A= 'Microsoft.XMLHTTP');=0A= for (var i =3D 0; i < msxmls.length; i++) {=0A= try {=0A= return new ActiveXObject(msxmls[i]);=0A= } catch (e) {=0A= }=0A= }=0A= return null;=0A= };=0A= }=0A= // Gecko support=0A= /* ;-) */=0A= // Opera support=0A= if (window.opera && !window.XMLHttpRequest) {=0A= window.XMLHttpRequest =3D function() {=0A= this.readyState =3D 0; 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i < this._headers.length; i++) {=0A= if (header =3D=3D '*' || this._headers[i].h =3D=3D header) {=0A= ret +=3D this._headers[i].h + ': ' + this._headers[i].v + '\n';=0A= }=0A= }=0A= return ret;=0A= };=0A= this.getResponseHeader =3D function(header) {=0A= var ret =3D getAllResponseHeader(header);=0A= var i =3D ret.indexOf('\n');=0A= if (i !=3D -1) {=0A= ret =3D ret.substring(0, i);=0A= }=0A= return ret;=0A= };=0A= this.setRequestHeader =3D function(header, value) {=0A= this._headers[this._headers.length] =3D {h:header, v:value};=0A= };=0A= this.open =3D function(method, url, async, user, password) {=0A= this.method =3D method;=0A= this.url =3D url;=0A= this._async =3D true;=0A= this._aborted =3D false;=0A= this._headers =3D [];=0A= if (arguments.length >=3D 3) {=0A= this._async =3D async;=0A= }=0A= if (arguments.length > 3) {=0A= opera.postError('XMLHttpRequest.open() - user/password not = supported');=0A= }=0A= this.readyState =3D 1;=0A= if (this.onreadystatechange) {=0A= this.onreadystatechange();=0A= }=0A= };=0A= this.send =3D function(data) {=0A= if (!navigator.javaEnabled()) {=0A= alert("XMLHttpRequest.send() - Java must be installed and = enabled.");=0A= return;=0A= }=0A= if (this._async) {=0A= setTimeout(this._sendasync, 0, this, data);=0A= // this is not really asynchronous and won't execute until the = current=0A= // execution context ends=0A= } else {=0A= this._sendsync(data);=0A= }=0A= }=0A= this._sendasync =3D function(req, data) {=0A= if (!req._aborted) {=0A= req._sendsync(data);=0A= }=0A= };=0A= this._sendsync =3D function(data) {=0A= this.readyState =3D 2;=0A= if (this.onreadystatechange) {=0A= this.onreadystatechange();=0A= }=0A= // open connection=0A= var url =3D new java.net.URL(new = java.net.URL(window.location.href), this.url);=0A= var conn =3D url.openConnection();=0A= for (var i =3D 0; i < this._headers.length; i++) {=0A= conn.setRequestProperty(this._headers[i].h, this._headers[i].v);=0A= }=0A= this._headers =3D [];=0A= if (this.method =3D=3D 'POST') {=0A= // POST data=0A= conn.setDoOutput(true);=0A= var wr =3D new = java.io.OutputStreamWriter(conn.getOutputStream(), this._getCharset());=0A= wr.write(data);=0A= wr.flush();=0A= wr.close();=0A= }=0A= // read response headers=0A= // NOTE: the getHeaderField() methods always return nulls for me :(=0A= var gotContentEncoding =3D false;=0A= var gotContentLength =3D false;=0A= var gotContentType =3D false;=0A= var gotDate =3D false;=0A= var gotExpiration =3D false;=0A= var gotLastModified =3D false;=0A= for (var i =3D 0; ; i++) {=0A= var hdrName =3D conn.getHeaderFieldKey(i);=0A= var hdrValue =3D conn.getHeaderField(i);=0A= if (hdrName =3D=3D null && hdrValue =3D=3D null) {=0A= break;=0A= }=0A= if (hdrName !=3D null) {=0A= this._headers[this._headers.length] =3D {h:hdrName, = v:hdrValue};=0A= switch (hdrName.toLowerCase()) {=0A= case 'content-encoding': gotContentEncoding =3D true; break;=0A= case 'content-length' : gotContentLength =3D true; break;=0A= case 'content-type' : gotContentType =3D true; break;=0A= case 'date' : gotDate =3D true; break;=0A= case 'expires' : gotExpiration =3D true; break;=0A= case 'last-modified' : gotLastModified =3D true; break;=0A= }=0A= }=0A= }=0A= // try to fill in any missing header information=0A= var val;=0A= val =3D conn.getContentEncoding();=0A= if (val !=3D null && !gotContentEncoding) = this._headers[this._headers.length] =3D {h:'Content-encoding', v:val};=0A= val =3D conn.getContentLength();=0A= if (val !=3D -1 && !gotContentLength) = this._headers[this._headers.length] =3D {h:'Content-length', v:val};=0A= val =3D conn.getContentType();=0A= if (val !=3D null && !gotContentType) = this._headers[this._headers.length] =3D {h:'Content-type', v:val};=0A= val =3D conn.getDate();=0A= if (val !=3D 0 && !gotDate) this._headers[this._headers.length] = =3D {h:'Date', v:(new Date(val)).toUTCString()};=0A= val =3D conn.getExpiration();=0A= if (val !=3D 0 && !gotExpiration) = this._headers[this._headers.length] =3D {h:'Expires', v:(new = Date(val)).toUTCString()};=0A= val =3D conn.getLastModified();=0A= if (val !=3D 0 && !gotLastModified) = this._headers[this._headers.length] =3D {h:'Last-modified', v:(new = Date(val)).toUTCString()};=0A= // read response data=0A= var reqdata =3D '';=0A= var stream =3D conn.getInputStream();=0A= if (stream) {=0A= var reader =3D new java.io.BufferedReader(new = java.io.InputStreamReader(stream, this._getCharset()));=0A= var line;=0A= while ((line =3D reader.readLine()) !=3D null) {=0A= if (this.readyState =3D=3D 2) {=0A= this.readyState =3D 3;=0A= if (this.onreadystatechange) {=0A= this.onreadystatechange();=0A= }=0A= }=0A= reqdata +=3D line + '\n';=0A= }=0A= reader.close();=0A= this.status =3D 200;=0A= this.statusText =3D 'OK';=0A= this.responseText =3D reqdata;=0A= this.readyState =3D 4;=0A= if (this.onreadystatechange) {=0A= this.onreadystatechange();=0A= }=0A= if (this.onload) {=0A= this.onload();=0A= }=0A= } else {=0A= // error=0A= this.status =3D 404;=0A= this.statusText =3D 'Not Found';=0A= this.responseText =3D '';=0A= this.readyState =3D 4;=0A= if (this.onreadystatechange) {=0A= this.onreadystatechange();=0A= }=0A= if (this.onerror) {=0A= this.onerror();=0A= }=0A= }=0A= };=0A= };=0A= }=0A= // ActiveXObject emulation=0A= if (!window.ActiveXObject && window.XMLHttpRequest) {=0A= window.ActiveXObject =3D function(type) {=0A= switch (type.toLowerCase()) {=0A= case 'microsoft.xmlhttp':=0A= case 'msxml2.xmlhttp':=0A= case 'msxml2.xmlhttp.3.0':=0A= case 'msxml2.xmlhttp.4.0':=0A= case 'msxml2.xmlhttp.5.0':=0A= return new XMLHttpRequest();=0A= }=0A= return null;=0A= };=0A= }=0A= ------=_NextPart_000_0000_01C85B72.C9DBE550 Content-Type: application/octet-stream Content-Transfer-Encoding: quoted-printable Content-Location: http://www.jneurosci.org/javascript/ajax/utility.js /************************************************************************= *****=0A= * javascript/ajax/utility.js=0A= *=0A= * Utility functions for working with XMLHttpRequest data.=0A= *=0A= * Copyright 2006 Board of Trustees of the Leland Stanford Junior = University.=0A= = *************************************************************************= ***/=0A= =0A= /*=0A= * Copy XML nodes into an HTMLElement. This effectively=0A= * clones XML markup which uses XHTML naming conventions=0A= * into an HTML DOM.=0A= */=0A= function copy_xml_to_html(src, dst) {=0A= if (src.nodeType =3D=3D 1) { /* Node.ELEMENT_NODE */=0A= var e =3D document.createElement(src.nodeName);=0A= for (var i =3D 0; i < src.childNodes.length; i++) {=0A= copy_xml_to_html(src.childNodes[i], e);=0A= }=0A= for (var i =3D 0; i < src.attributes.length; i++) {=0A= var n =3D src.attributes[i].name;=0A= var v =3D unescape_xml_string(src.attributes[i].value); =0A= e.setAttribute(n, v);=0A= if (n =3D=3D "class") {=0A= e.className =3D v;=0A= }=0A= else if (n =3D=3D "style") {=0A= set_css_style(v, e, "");=0A= }=0A= }=0A= dst.appendChild(e);=0A= }=0A= else if (src.nodeType =3D=3D 3) { /* Node.TEXT_NODE */=0A= dst.appendChild(document.createTextNode(src.nodeValue));=0A= }=0A= }=0A= =0A= /* =0A= * It is unclear that this is the right thing to be calling=0A= * from copy_xml_to_html, but it appears that Safari decides=0A= * to convert & to the NCR #, and then encodes that=0A= * NCR to &%26%2338;. So, I'm going to treat the DOM Attr=0A= * value as a plain string, and run our XML string input=0A= * through the decoding routine below.=0A= */=0A= function unescape_xml_string(s) {=0A= return s.replace(/'/g, "'")=0A= .replace(/'/g, "'")=0A= .replace(/"/g, "\"")=0A= .replace(/"/g, "\"")=0A= .replace(/>/g, ">")=0A= .replace(/>/g, ">")=0A= .replace(/</g, "<")=0A= .replace(/</g, "<")=0A= .replace(/&/g, "&")=0A= .replace(/&/g, "&");=0A= }=0A= =0A= /*=0A= * Parse set of CSS rules and apply them to an element.=0A= * This is quite horrifying, but I'm unable to determine=0A= * how else to handle this with IE 6. FireFox and other=0A= * sane browsers let you simply set the style attribute=0A= * or use e.style.setProperty(rule, value, priority),=0A= * IE 6 appears to have neither of these capabilities..=0A= */=0A= function set_css_style(css, e, priority) {=0A= var rules =3D css.split(";");=0A= for (var i =3D 0; i < rules.length; i++) {=0A= var nvpair =3D rules[i].split(":");=0A= if (nvpair.length =3D=3D 2) {=0A= try {=0A= var name =3D nvpair[0]; /* style attribute */=0A= var value =3D nvpair[1]; /* attribute value */=0A= =0A= /*=0A= * For each possible style attribute, set the=0A= * appropriate style property in the element.=0A= */=0A= if (name =3D=3D "background") {=0A= e.style.background =3D value;=0A= }=0A= else if (name =3D=3D "background-attachment") {=0A= e.style.backgroundAttachment =3D value;=0A= }=0A= else if (name =3D=3D "background-color") {=0A= e.style.backgroundColor =3D value;=0A= }=0A= else if (name =3D=3D "background-image") {=0A= e.style.backgroundImage =3D value;=0A= }=0A= else if (name =3D=3D "background-position") {=0A= e.style.backgroundPosition =3D value;=0A= }=0A= else if (name =3D=3D "background-position-x") {=0A= e.style.backgroundPositionX =3D value;=0A= }=0A= else if (name =3D=3D "background-position-y") {=0A= e.style.backgroundPositionY =3D value;=0A= }=0A= else if (name =3D=3D "background-repeat") {=0A= e.style.backgroundRepeat =3D value;=0A= }=0A= else if (name =3D=3D "behavior") {=0A= e.style.behavior =3D value;=0A= }=0A= else if (name =3D=3D "border") {=0A= e.style.border =3D value;=0A= }=0A= else if (name =3D=3D "border-bottom") {=0A= e.style.borderBottom =3D value;=0A= }=0A= else if (name =3D=3D "border-bottom-color") {=0A= e.style.borderBottomColor =3D value;=0A= }=0A= else if (name =3D=3D "border-bottom-style") {=0A= e.style.borderBottomStyle =3D value;=0A= }=0A= else if (name =3D=3D "border-bottom-width") {=0A= e.style.borderBottomWidth =3D value;=0A= }=0A= else if (name =3D=3D "border-collapse") {=0A= e.style.borderCollapse =3D value;=0A= }=0A= else if (name =3D=3D "border-color") {=0A= e.style.borderColor =3D value;=0A= }=0A= else if (name =3D=3D "border-left") {=0A= e.style.borderLeft =3D value;=0A= }=0A= else if (name =3D=3D "border-left-color") {=0A= e.style.borderLeftColor =3D value;=0A= }=0A= else if (name =3D=3D "border-left-style") {=0A= e.style.borderLeftStyle =3D value;=0A= }=0A= else if (name =3D=3D "border-left-width") {=0A= e.style.borderLeftWidth =3D value;=0A= }=0A= else if (name =3D=3D "border-right") {=0A= e.style.borderRight =3D value;=0A= }=0A= else if (name =3D=3D "border-right-color") {=0A= e.style.borderRightColor =3D value;=0A= }=0A= else if (name =3D=3D "border-right-style") {=0A= e.style.borderRightStyle =3D value;=0A= }=0A= else if (name =3D=3D "border-right-width") {=0A= e.style.borderRightWidth =3D value;=0A= }=0A= else if (name =3D=3D "border-style") {=0A= e.style.borderStyle =3D value;=0A= }=0A= else if (name =3D=3D "border-top") {=0A= e.style.borderTop =3D value;=0A= }=0A= else if (name =3D=3D "border-top-color") {=0A= e.style.borderTopColor =3D value;=0A= }=0A= else if (name =3D=3D "border-top-style") {=0A= e.style.borderTopStyle =3D value;=0A= }=0A= else if (name =3D=3D "border-top-width") {=0A= e.style.borderTopWidth =3D value;=0A= }=0A= else if (name =3D=3D "border-width") {=0A= e.style.borderWidth =3D value;=0A= }=0A= else if (name =3D=3D "bottom") {=0A= e.style.bottom =3D value;=0A= }=0A= else if (name =3D=3D "clear") {=0A= e.style.clear =3D value;=0A= }=0A= else if (name =3D=3D "clip") {=0A= e.style.clip =3D value;=0A= }=0A= else if (name =3D=3D "color") {=0A= e.style.color =3D value;=0A= }=0A= else if (name =3D=3D "cssText") {=0A= e.style.Sets =3D value;=0A= }=0A= else if (name =3D=3D "cursor") {=0A= e.style.cursor =3D value;=0A= }=0A= else if (name =3D=3D "direction") {=0A= e.style.direction =3D value;=0A= }=0A= else if (name =3D=3D "display") {=0A= e.style.display =3D value;=0A= }=0A= else if (name =3D=3D "font") {=0A= e.style.font =3D value;=0A= }=0A= else if (name =3D=3D "font-family") {=0A= e.style.fontFamily =3D value;=0A= }=0A= else if (name =3D=3D "font-size") {=0A= e.style.fontSize =3D value;=0A= }=0A= else if (name =3D=3D "font-style") {=0A= e.style.fontStyle =3D value;=0A= }=0A= else if (name =3D=3D "font-variant") {=0A= e.style.fontVariant =3D value;=0A= }=0A= else if (name =3D=3D "font-weight") {=0A= e.style.fontWeight =3D value;=0A= }=0A= else if (name =3D=3D "height") {=0A= e.style.height =3D value;=0A= }=0A= else if (name =3D=3D "ime-mode") {=0A= e.style.imeMode =3D value;=0A= }=0A= else if (name =3D=3D "layout-flow") {=0A= e.style.layoutFlow =3D value;=0A= }=0A= else if (name =3D=3D "layout-grid") {=0A= e.style.layoutGrid =3D value;=0A= }=0A= else if (name =3D=3D "layout-grid-char") {=0A= e.style.layoutGridChar =3D value;=0A= }=0A= else if (name =3D=3D "layout-grid-line") {=0A= e.style.layoutGridLine =3D value;=0A= }=0A= else if (name =3D=3D "layout-grid-mode") {=0A= e.style.layoutGridMode =3D value;=0A= }=0A= else if (name =3D=3D "layout-grid-type") {=0A= e.style.layoutGridType =3D value;=0A= }=0A= else if (name =3D=3D "left") {=0A= e.style.left =3D value;=0A= }=0A= else if (name =3D=3D "letter-spacing") {=0A= e.style.letterSpacing =3D value;=0A= }=0A= else if (name =3D=3D "line-break") {=0A= e.style.lineBreak =3D value;=0A= }=0A= else if (name =3D=3D "line-height") {=0A= e.style.lineHeight =3D value;=0A= }=0A= else if (name =3D=3D "list-style") {=0A= e.style.listStyle =3D value;=0A= }=0A= else if (name =3D=3D "list-style-image") {=0A= e.style.listStyleImage =3D value;=0A= }=0A= else if (name =3D=3D "list-style-position") {=0A= e.style.listStylePosition =3D value;=0A= }=0A= else if (name =3D=3D "list-style-type") {=0A= e.style.listStyleType =3D value;=0A= }=0A= else if (name =3D=3D "margin") {=0A= e.style.margin =3D value;=0A= }=0A= else if (name =3D=3D "margin-bottom") {=0A= e.style.marginBottom =3D value;=0A= }=0A= else if (name =3D=3D "margin-left") {=0A= e.style.marginLeft =3D value;=0A= }=0A= else if (name =3D=3D "margin-right") {=0A= e.style.marginRight =3D value;=0A= }=0A= else if (name =3D=3D "margin-top") {=0A= e.style.marginTop =3D value;=0A= }=0A= else if (name =3D=3D "min-height") {=0A= e.style.minHeight =3D value;=0A= }=0A= else if (name =3D=3D "overflow") {=0A= e.style.overflow =3D value;=0A= }=0A= else if (name =3D=3D "overflow-x") {=0A= e.style.overflowX =3D value;=0A= }=0A= else if (name =3D=3D "overflow-y") {=0A= e.style.overflowY =3D value;=0A= }=0A= else if (name =3D=3D "padding") {=0A= e.style.padding =3D value;=0A= }=0A= else if (name =3D=3D "padding-bottom") {=0A= e.style.paddingBottom =3D value;=0A= }=0A= else if (name =3D=3D "padding-left") {=0A= e.style.paddingLeft =3D value;=0A= }=0A= else if (name =3D=3D "padding-right") {=0A= e.style.paddingRight =3D value;=0A= }=0A= else if (name =3D=3D "padding-top") {=0A= e.style.paddingTop =3D value;=0A= }=0A= else if (name =3D=3D "page-break-after") {=0A= e.style.pageBreakAfter =3D value;=0A= }=0A= else if (name =3D=3D "page-break-before") {=0A= e.style.pageBreakBefore =3D value;=0A= }=0A= else if (name =3D=3D "pixelBottom") {=0A= e.style.pixelBottom =3D value;=0A= }=0A= else if (name =3D=3D "pixelHeight") {=0A= e.style.pixelHeight =3D value;=0A= }=0A= else if (name =3D=3D "pixelLeft") {=0A= e.style.pixelLeft =3D value;=0A= }=0A= else if (name =3D=3D "pixelRight") {=0A= e.style.pixelRight =3D value;=0A= }=0A= else if (name =3D=3D "pixelTop") {=0A= e.style.pixelTop =3D value;=0A= }=0A= else if (name =3D=3D "pixelWidth") {=0A= e.style.pixelWidth =3D value;=0A= }=0A= else if (name =3D=3D "posBottom") {=0A= e.style.posBottom =3D value;=0A= }=0A= else if (name =3D=3D "posHeight") {=0A= e.style.posHeight =3D value;=0A= }=0A= else if (name =3D=3D "position") {=0A= e.style.position =3D value;=0A= }=0A= else if (name =3D=3D "posLeft") {=0A= e.style.posLeft =3D value;=0A= }=0A= else if (name =3D=3D "posRight") {=0A= e.style.posRight =3D value;=0A= }=0A= else if (name =3D=3D "posTop") {=0A= e.style.posTop =3D value;=0A= }=0A= else if (name =3D=3D "posWidth") {=0A= e.style.posWidth =3D value;=0A= }=0A= else if (name =3D=3D "right") {=0A= e.style.right =3D value;=0A= }=0A= else if (name =3D=3D "ruby-align") {=0A= e.style.rubyAlign =3D value;=0A= }=0A= else if (name =3D=3D "ruby-overhang") {=0A= e.style.rubyOverhang =3D value;=0A= }=0A= else if (name =3D=3D "ruby-position") {=0A= e.style.rubyPosition =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-3dlight-color") {=0A= e.style.scrollbar3dLightColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-arrow-color") {=0A= e.style.scrollbarArrowColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-base-color") {=0A= e.style.scrollbarBaseColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-darkshadow-color") {=0A= e.style.scrollbarDarkShadowColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-face-color") {=0A= e.style.scrollbarFaceColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-highlight-color") {=0A= e.style.scrollbarHighlightColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-shadow-color") {=0A= e.style.scrollbarShadowColor =3D value;=0A= }=0A= else if (name =3D=3D "scrollbar-track-color") {=0A= e.style.scrollbarTrackColor =3D value;=0A= }=0A= else if (name =3D=3D "float") {=0A= e.style.styleFloat =3D value;=0A= }=0A= else if (name =3D=3D "table-layout") {=0A= e.style.tableLayout =3D value;=0A= }=0A= else if (name =3D=3D "text-align") {=0A= e.style.textAlign =3D value;=0A= }=0A= else if (name =3D=3D "text-align-last") {=0A= e.style.textAlignLast =3D value;=0A= }=0A= else if (name =3D=3D "text-autospace") {=0A= e.style.textAutospace =3D value;=0A= }=0A= else if (name =3D=3D "text-decoration") {=0A= e.style.textDecoration =3D value;=0A= }=0A= else if (name =3D=3D "textDecorationBlink") {=0A= e.style.textDecorationBlink =3D value;=0A= }=0A= else if (name =3D=3D "textDecorationLineThrough") {=0A= e.style.textDecorationLineThrough =3D value;=0A= }=0A= else if (name =3D=3D 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