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Effects
of N-6 essential fatty acids on glioma invasion and growth: experimental studies
with glioma spheroids in collagen gels
Bell
HS, Wharton SB, Leaver HA, Whittle IR
Department
of Clinical Neurosciences, Western General Hospital, Edinburgh, Scotland
Object.
Intracranial infusions of gamma-linolenic acid (GLA), an essential fatty acid,
have been used as an adjuvant therapy following malignant glioma resection;
however, little is known about the dose response of glioma cells to this
therapy.
In this in vitro study the authors address this important pharmacological
question.
Methods.
Glioma spheroids derived from U87, U373, MOG-G-CCM, and C6 cell lines were grown
in collagen gel and exposed to a range of GLA concentrations (0-1 mM) for 5
days.
The diameter of glioma spheroids was measured, the apoptotic index was assessed
using both the terminal deoxynucleotidyl transferase-mediated deoxyuridine
triphosphate nick-end labeling technique and cell morphological testing, and the
levels of proliferating cell nuclear antigen were also measured.
Conclusions.
The dose-response patterns were similar for all four glioma spheroids.
Low concentrations of GLA (<100 microM) increased both apoptosis and
proliferation with a net increase in tumor growth and invasion, whereas
high-dose GLA (>100 microM) significantly impaired spheroid cell growth.
The proliferative effects of low-dose GLA could be a hazard in the clinical
treatment of malignant glioma; however, because of the low toxicity of GLA
against normal cells, local delivery of millimolar doses of GLA could
significantly reduce tumor size.
PMID:
10584845 [PubMed - indexed for MEDLINE]
Source:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10584845&dopt=Abstract
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