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Inhibition
of angiogenic factor- and tumour-induced angiogenesis by gamma linolenic acid
Cai
J, Jiang WG, Mansel RE
Metastasis
Research Group, Department of Surgery, University of Wales College of Medicine,
Health Park, Cardiff, UK
Angiogenesis,
the formation of new blood vessels, is an essential feature of malignant tumour
development.
Gamma linolenic acid (GLA), a n-6 polyunsaturated fatty acid (PUFA), inhibits
the growth and metastasis of a variety of tumour cells, including breast,
prostate, pancreatic cancer and hepatoma cells and also has anti-metastatic
effects on endothelial cells.
In the current study, we tested whether GLA inhibited angiogenesis induced by
tumour cells.
A rat aortic ring assay and in vitro tube formation of human vascular
endothelial cells were used to determine angiogenesis (spontaneous, angiogenic
factor- and tumour cells-induced).
Inclusion of GLA in this 3-D matrix culture system significantly inhibited
angiogenesis from aortic rings in a concentration-dependent manner.
The results from tube formation of vascular endothelial cell further confirmed
that GLA suppressed angiogenesis.
Furthermore, in the cell motility assay (phagokinetic assay and endothelial
wounding assay), a significant reduction of the motility of vascular endothelial
cells by GLA was seen.
It is concluded that gamma linolenic acid inhibits angiogenic factor and
tumour-induced angiogenesis in vitro at least in part via its inhibitory effect
on the motility of vascular endothelial cells.
PMID:
10319914 [PubMed - indexed for MEDLINE]
Source:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10319914&dopt=Abstract
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