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Selenium
causes growth inhibition and apoptosis in human brain tumor cell lines
Sundaram
N, Pahwa AK, Ard MD, Lin N, Perkins E, Bowles AP Jr
Department
of Neurosurgery, University of Mississippi Medical Center, Jackson 39216-4505,
USA
We
examined the effect of the trace element selenium on human glioma cell lines:
T98G, U373MG, and U87MG, in addition to dermal fibroblast cells.
Cultures were incubated with sodium selenite, and the following parameters were
studied: cell growth, mitochondrial function, and ultrastructure.
Cell growth was assayed by counting the number of viable cells after treatment
with selenium.
Mitochondrial function was analyzed using the MTT (tetrazolium salt reduction)
assay.
Apoptosis was determined by evaluating nuclear chromatin condensation by
electron microscopy.
The results indicated that selenium had a significant inhibitory effect on the
growth of the tumor cells but had little effect upon dermal fibroblasts which
had been passaged numerous times.
Selenium also induced mitochondrial damage as shown by MTT assay in two brain
tumor cell lines and in minimally passaged fibroblasts, but it had little effect
upon the high-passage fibroblasts.
Ultrastructurally, mitochondria had electron-dense inclusions resulting from
selenium treatment.
High rates of apoptosis were induced by selenium in the tumor cell lines and in
the minimally passaged fibroblasts, whereas the fibroblasts with a high number
of passages had some resistance to selenium treatment.
This study correlates the adverse effects of selenium on mitochondrial function,
inhibition of cell growth, and apoptosis and shows that selenium similarly
affects three different brain tumor cell lines and minimally passaged
fibroblasts.
Further, the results with fibroblasts show that some types of cells after
repeated passages can develop resistance to selenium damage.
PMID:
10894365 [PubMed - indexed for MEDLINE]
Source:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10894365&dopt=Abstract
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