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[Growth
inhibition of epidermal growth factor-stimulated human glioblastoma cells by
nicardipine in vitro]
[Article in Chinese]
Huang CX, Chen B, Zhang YF
Department of Neurosurgery, Second Xiangya Hospital, Central
South University, Changsha 410011, China
Objective. To study the growth effects of Epidermal Growth Factor (EGF)
and the calcium channel antagonist nicardipine (NC) on U251 MG, a human
malignant glioma cell lime, which has high-affinity EGF receptors.
Methods.
The growth effects of EGF and NC on U251 MG cultured in serum-free and
serum-containing (10% fetal bovine serum, FBS) media respectively were observed
by MTT colorimeritric analysis.
Results.
(1) EGF significantly enhanced the growth of U251 MG in a dose-dependent manner
in serum-free conditions.
The near-maximal effect was obtained at 10 mg.ml-1.
The addition of serum obscured this effect.
(2) NC decreased U251 MG cells proliferation, especially in serum-containing
media, and completely blocked the growth-stimulated effect of EGF.
The combinated effects of EGF (10 ng.ml-1) and NC equaled those of NC
alone.
Conclusions.
When serum is absent U251 MG cells showed a pronounced mitogenic response to EGF
in a dose dependent manner, which approximated that achieved with 10% FBS.
NC suppressed the growth of U251 MG cells and completely blocked the
growth-stimulated effects of EGF; it can be used as an adjuvant therapy for
human malignant gliomas.
PMID: 12536683 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12536683&dopt=Abstract |
