|
|
Bromelain
reversibly inhibits invasive properties of glioma cells
Tysnes BB, Maurer HR, Porwol T, Probst B, Bjerkvig R, Hoover
F
Department of Anatomy and Cell Biology, University of Bergen, Bergen N-5009,
Norway.
bert.tysnes@pki.uib.no
Bromelain is an aqueous extract from pineapple stem that contains proteinases
and exhibits pleiotropic therapeutic effects, i.e., antiedematous,
antiinflammatory, antimetastatic, antithrombotic, and fibrinolytic activities.
In this study, we tested bromelain's effects on glioma cells to assess whether
bromelain could be a potential contributor to new antiinvasive strategies for
gliomas. Several complementary assays demonstrated that bromelain significantly
and reversibly reduced glioma cell adhesion, migration, and invasion without
affecting cell viability, even after treatment periods extending over several
months.
Immunohistochemistry and immunoblotting experiments demonstrated that
alpha3 and beta1 integrin subunits and hyaluronan receptor CD44 protein levels
were reduced within 24 hours of bromelain treatment.
These effects were not
reflected at the RNA level because RNA profiling did not show any significant
effects on gene expression.
Interestingly, metabolic labelling with 35-S
methionine demonstrated that de novo protein synthesis was greatly attenuated by
bromelain, in a reversible manner.
By using a transactivating signaling assay,
we found that CRE-mediated signaling processes were suppressed.
These results
indicate that bromelain exerts its antiinvasive effects by proteolysis,
signaling cascades, and translational attenuation.
PMID: 11774029 [PubMed - indexed for MEDLINE]
Abstract
| 
Full
Text | 
Reprint
|
