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Curcumin
inhibits interleukin 8 production and enhances interleukin 8 receptor expression
on the cell surface: impact on human pancreatic carcinoma cell growth by
autocrine regulation
Hidaka H, Ishiko T, Furuhashi T, Kamohara H, Suzuki S, Miyazaki M,
Ikeda O, Mita S, Setoguchi T, Ogawa M
Department of Surgery I, Miyazaki Medical College, Japan
Background. Curcumin, the yellow pigment in turmeric, has been shown to
prevent tumor progression in a variety of tissues in rodents.
The authors investigated the effect of curcumin on human carcinoma cell lines to
determine whether constitutive interleukin-8 (IL-8) production of tumor cells
was correlated with nuclear factor kappaB (NF-kappaB) activation and cell growth
activity.
Methods. A human pancreatic carcinoma cell line, SUIT-2, was incubated
with various concentrations of curcumin for 2 hours.
Biologic features, including IL-8 production, DNA binding activity,
transactivation of NF-kappaB, cell growth activity, cell viability, and the
expression of IL-8 receptors (CXCR1 and CXCR2) were analyzed.
Results. The constitutive production of IL-8 was inhibited by curcumin at
concentrations of 10-100 microM in a dose dependent manner.
NF-kappaB activity was reduced significantly by curcumin treatment.
Pretreatment with curcumin inhibited the growth rate of carcinoma cells
significantly.
Such cell growth inhibition by curcumin was not recovered by exogenous
recombinant IL-8.
The investigation of expression in IL-8 receptors, CXCR1 and CXCR2, revealed
that the expression of both receptors was enhanced remarkably by curcumin.
Exogenous IL-8 could not recover this enhancement of IL-8 receptors.
These results suggest that curcumin inhibits IL-8-induced receptor
internalization.
Conclusions. The authors concluded that curcumin contributed not only to
the inhibition of IL-8 production but also to signal transduction through IL-8
receptors. These data suggest that curcumin reduces numerous IL-8 bioactivities that
contribute to tumor growth and carcinoma cell viability.
From this point of view, curcumin is a potent anticancer agent that inhibits the
production of proinflammatory cytokines, including IL-8, by tumor cells.
Copyright 2002 American Cancer Society.
PMID: 12216086 [PubMed - indexed for MEDLINE]
Source:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12216086&dopt=Abstract
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