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Inhibition
of proliferation and induction of differentiation of glioma cells with Datura
stramonium agglutinin
Sasaki T,
Yamazaki K, Yamori T, Endo T
Department
of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho,
Itabashi-ku, Tokyo 173-0015, Japan
We found
that a lectin, Datura stramonium agglutinin, induced irreversible
differentiation in C6 glioma cells.
The differentiated cells had long processes, a low rate of proliferation and a
high content of glial fibrillary acidic protein.
When the medium was replaced with Datura stramonium agglutinin-free medium after
1 h, cell proliferation continued to be inhibited.
Experiments with several other lectins indicated that both recognition of linear
N-acetyllactosamine repeats and recognition of multiantennary units of
cell-surface glycans were required for the inhibition of C6 proliferation.
Proliferation of four human glial tumour cells was also inhibited by Datura
stramonium agglutinin.
Further, these differentiated human glial tumour cells had long processes and a
high content of glial fibrillary acidic protein similar to differentiated C6
glioma cells.
Taken together, these observations suggest that Datura stramonium agglutinin may
be useful as a new therapy for treating glioma without side effects.
Copyright 2002 Cancer Research UK
PMID:
12373609 [PubMed - in process]
Source:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12373609&dopt=Abstract
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