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Effects
of Thalidomide on Parameters Involved in Angiogenesis: An in vitro
Study
M. Gelati, E. Corsini, S. Frigerio,
B. Pollo, G. Broggi, D. Croci, A. Silvani, A. Boiardi, A. Salmaggi
Istituto Nazionale Neurologico 'C.Besta',
Milan, Italy
In an
attempt to elucidate the mechanism(s) of action of thalidomide, a reportedly
antiangiogenic molecule recently tested in the treatment of relapsing malignant
gliomas, we performed an in vitro study on the following
parameters:
(a) effect of thalidomide on proliferation of endothelial cells;
(b) effect of thalidomide on expression of αvβ3
integrin on the surface of endothelial cells;
(c) effect of thalidomide on the release by endothelial cells of MMP-2, IL-8 and
TNF-α.
The results show that thalidomide inhibits endotelial cell proliferation induced
by bFGF and VEGF, more so if the cells are grown on vitronectin; moreover,
treatment with thalidomide reduces the release of MMP-2 and IL-8 by endothelial
cells, suggesting a further pathway for the antiangiogenic activity of
drug.
On the other hand, thalidomide does not modify expression of αvβ3
on endothelial cells.
Copyright
© 2003 Kluwer
Academic Publishers. All rights reserved
Source: http://www.kluweronline.com/issn/0167-594X/contents
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