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Medulloblastoma
cell invasion is inhibited by green tea (-)epigallocatechin-3-gallate
Pilorget A, Berthet V,
Luis J, Moghrabi A, Annabi B, Beliveau R
Laboratoire de Medecine Moleculaire, Centre de Cancerologie
Charles Bruneau, Hopital Sainte-Justine and Universite du Quebec a Montreal,
Montreal, Quebec, Canada.
Epigallocatechin-3-gallate
(EGCG), the major green tea polyphenol, can reach the brain following oral
intake and could thus act as an anti-tumoral agent targeting several key steps
of brain cancer cells invasive activity.
Because integrin-mediated extracellular matrix recognition is crucial during the
cell adhesion processes involved in carcinogenesis, we have investigated the
effects of EGCG on different cellular integrins of the pediatric brain
tumor-derived medulloblastoma cell line DAOY.
Using flow cytometry, we report the levels of expression of several cell surface
integrins in DAOY.
These include high expression of alpha2, alpha3, and beta1 integrins, as well as
alphav and beta3 integrins.
Moreover, we provide evidence that EGCG can antagonize DAOY cell migration
specifically on collagen by increasing cell adhesive ability through specific
gene and protein upregulation of the beta1 integrin subunit.
Our results suggest that this naturally occurring green tea polyphenol may thus
be used as a nutraceutical therapeutic agent in targeting the invasive character
of medulloblastomas.
Copyright 2003 Wiley-Liss, Inc.
PMID: 14587030 [PubMed - in process]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14587030&dopt=Abstract
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