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In vivo tracking of neural progenitor cell migration to
glioblastomas
Tang Y, Shah K, Messerli SM, Snyder E, Breakefield X, Weissleder R.
Center for Molecular Imaging Research, Department of Radiology, Massachusetts
General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
The ability to noninvasively track the migration, engraftment, and proliferation
of neural progenitor cells (NPCs) has significant clinical and research
implications.
The purpose of our study was to explore the macroscopic migratory
capabilities of NPCs toward brain tumors after implantation into nude mice.
We
stably transfected C17.2 NPCs with the firefly luciferase gene (F-luc) and
implanted cells into
(1) the contralateral brain parenchyma (2 x 10(6) cells),
(2) the ventricles (2 x 10(6) cells),
(3) the vasculature (1 x 10(5) cells), or
(4) the intraperitoneal cavity (5 x 10(6) cells) of mice bearing intracranial
gliomas (Gli36).
Using serial bioluminescence imaging, migration of
parenchymally injected cells was observed across the corpus callosum, first
detected at 1 week, with maximal density at the tumor site 2-3 weeks after
implantation.
Similar patterns were also observed with intraventricular
injections; however, tumors were populated earlier, presumably because of the
shorter distance to travel.
Intravenous injections resulted in more modest
tumoral NPC populations, whereas virtually no cells could be identified in
tumors after intraperitoneal injection. These results confirm the migratory
capability of NPCs over considerable distances and their preferential
accumulation in brain tumors on CNS rather than peripheral injection.
PMID: 12952596 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12952596
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