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Expression of somatostatin receptor mRNA in human meningiomas
and their implication in in
vitro antiproliferative activity
Sara Arena,
Federica Barbieri,
Stefano Thellung, Paolo Pirani, Alessandro Corsaro, Valentina Villa,
Patrizia Dadati, Alessandra Dorcaratto, Gabriella Lapertosa, Jean-Louis Ravetti, Renato
Spaziante, Gennaro Schettini
and Tullio Florio
Section of
Pharmacology, Department of Oncology, Biology and Genetics (S.A., S.T., A,C.,
V.V., G.S., T.F.), Section
of Neurosurgery DISCAT (A.D.) , Section
of Pathology, DICMI (G.L.), Division
of Neurosurgery, Department of Neuroscience, Opthalmology and Genetics,
University of Genova (R.S.); Pharmacology
and Neurosciences, National Institute for Cancer Research (IST), Genova (F.B.,
P.P., G.S., T.F.); Section
of Pathology, San Martino Hospital, Genova, Italy (P.D., J.-L.R.)
Somatostatin receptors (SSTRs)
have been detected in many normal and malignant tissues.
This wide expression has been used for
diagnostic, prognostic and therapeutic purposes.
Five SSTR subtypes (SSTR 1–5) have been identified
whose activation is responsible for the signal transduction through many
different intracellular pathways.
In the present study the expression of SSTR mRNA was determined by
reverse-transcriptase (RT)–PCR in 42 meningiomas.
About 88% of the tumors analyzed (37/42) were positive for at least one of the
five SSTR subtypes displaying a
variable pattern of expression of the different SSTR subtypes.
SSTR1 and SSTR2 were the most frequently
mRNA detected (69% and 79% of the sample analyzed, respectively).
The other subtypes were found in the
43%, 33% and 33% of cases for SSTR3, SSTR4 and SSTR5, respectively.
In 22, out of 42 patients (52%) three or
more SSTRs were detected.
The expression of the different SSTR subtypes did not correlate with the
expression of bcl-2
(apoptosis-associated protein) and MIB-1 (a proliferation marker), assessed by
immunohistochemistry in a series
of 34 tumor samples, while a correlation between the expression of SSTR3 and p53
was observed (p = 0.08).
To evaluate a possible role of SSTR in the control of human meningioma cell
proliferation, seven primary cell specimens
we analyzed altogether with in vitro antiproliferative
effects of SST may open interesting perspectives for
the diagnosis and the therapy of meningiomas.
Key words: meningioma,
primary cultures, proliferation, RT–PCR, somatostatin receptors
© 2004 Kluwer
Academic Publishers. Printed in the Netherlands.
Source: http://journals.kluweronline.com/article.asp?PIPS=5149479
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