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Accumulation of Boron in Human Malignant
Glioma Cells in vitro is Cell Type Dependent
Maria Dahlström, Jacek Capala, Peter Lindström, Åke
Wasteson, Annelie Lindström
Division
of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health
Sciences, Linköping University, Sweden (M.D.,
Å.W. A.L.);
Studsvik Medical AB, Sweden; Unit of Biomedical
Radiation Sciences, Uppsala University, Sweden (J.C.);
Department of Physics and Measurement Technology, Linköping
Institute of Technology, Linköping University, Sweden (P.L.)
It has been shown that human malignant glioma tumours consist of several
subpopulations of tumour cells.
Due to heterogeneity and different degrees of vascularisation cell
subpopulations possess varying resistance to chemo- or radiation therapy.
Therefore, therapy is dependent on the ability to specifically target a
tumour cell.
Boron neutron capture therapy (BNCT) is a bimodal method, in radiation
therapy, taking advantage of the ability of the stable isotope boron-10 to
capture neutrons.
It results in disintegration products depositing large amounts of energy
within a short length, approximately one cell diameter.
Thereby, selective irradiation of a target cell may be accomplished if a
sufficient amount of boron has been accumulated and hence the cell-associated
boron concentration is of critical importance.
The accumulation of boron, boronophenylalanine (BPA), was investigated in
two human glioma cell subpopulations and a human fibroblast cell line in
vitro.
The cells were incubated at low boron concentrations (0–5
μg
B/ml).
Oil
filtration was then used for separation of extracellular and cell-associated
boron.
Inductively
coupled plasma atomic emission spectroscopy (ICP-AES) was used for boron
determination.
Significant
(P < 0.05) differences in accumulation ratio (relation between
cell-associated and extracellular boron concentration) between human malignant
glioma cell lines were found.
Human
fibroblasts, used to represent normal cells, showed a growth-dependent uptake
and a lower accumulation ratio than the glioma cells.
Our
findings indicate that BPA concentration, incubation time and differences in
boron uptake between cell subpopulations should be considered in BNCT.
Keywords: BNCT,
BPA, fibroblasts, glioblastoma multiforme, glioma cell lines, in vitro
Copyright
©
2004 Kluwer Academic Publishers.
All rights reserved
Source: http://ipsapp009.kluweronline.com/IPS/content/ext/x/J/5042/I/119/A/2/abstract.htm
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