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Parent
Phenotype and Age Dependence, on Rat Glioma Tumor Rejection
Alicia Gonzalez-Martin, Daniel Muñoz-Espín, Andrés
M. Alonso, Marta Izquierdo
Departamento
de Biología Molecular-Centro de Biología Molecular Severo Ochoa [A.G.-M., D.M.-E.].
Departamento de Matemáticas, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain [A.M.A.]. Departamento de
Biología Molecular-Centro de Biología Molecular Severo Ochoa; Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain; Tel.- +34-91497-4857; Fax- +34-91497-4799; E-mail: mizquierdo@cbm.uam.es
[M.I.].
Background.
The
brain, despite the blood-brain barrier, does not escape to the highly variable
host rejection response mediated by a very strong and complex immune reaction
when rat glioma cells are transplanted into the adult animal.
Methods.
Crosses were performed among parents that are able or enable to reject a
well-known brain tumor cell line (C6).
Newborn animals were also challenged with rat glioma cells both in the brain and
the side flanks.
Results.
The
percentage of susceptibility or resistance to develop a lethal glioma can be
estimated knowing the parental phenotypes.
When both parents had rejected an induced tumor, 63% of the progeny will also
reject it.
Similarly, if both parents died as a consequence of the tumor, 70% of the
progeny would also be unable to reject the challenge of glioma C6 cells.
Newborn animals do not have a mature immune system and they tolerate
transplanted cells much better than adults.
We found no rejection to glioma C6, at both brain and side flank sites, in
1-day-old neonatal Wistar rats.
Tumors were beginning to be eliminated if the cells are inoculated at day 3 from
birth on the flanks, and at 1week from birth on the brain.
Conclusions.
There
is a genetic component conferring susceptibility or resistance to the lethal
effect of tumor development and progression depending on the parental phenotype
of the adult rats.
Neonatal rats
represent a much more reliable model than adults to study experimental therapies
against gliomas.
Keywords: glioma,
host rejection, neonatal rats, parental phenotype, tumor transplantation
Copyright
©
2004 Kluwer Academic Publishers.
All rights reserved
Source: http://ipsapp009.kluweronline.com/IPS/content/ext/x/J/5042/I/123/A/13/abstract.htm
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