|
|
Irradiation and Taxol treatment result in
non-monotonous, dose-dependent changes in the motility of glioblastoma cells
Balázs Hegedüs, Júlia Zách, András
Czirók, József Lövey and Tamás Vicsek
Department of
Biological Physics, Eötvös University (B.H., J.Z. A.C. T.V.), National
Institute of Neurosurgery (B.H.), Department of
Radiotherapy, National Institute of Oncology, Budapest, Hungary (J.L.),
Department of Cell Biology and Anatomy,
University of Kansas Medical Center, Kansas City, KS, USA (A.C.). hebal@biol-phys.elte.hu
Objective. Three-dimensional conformal radiotherapy, stereotactic
radiosurgery and concurrent chemoradiotherapy are among the most important
postoperative therapeutic measures in the treatment of malignant gliomas.
We investigated in vitro how these modalities affect cell motility, a key factor
in tumor invasiveness and malignancy.
Methods. A highly motile glioblastoma cell line was exposed to
clinically relevant (2-20 Gy) radiation doses.
Some cultures were also subjected to radiosensitizing treatment, in which 5 and
10 nM Taxol was added to the medium for 2 h before the irradiation.
The surviving cell fraction was continuously monitored during a 3 day-long time
period using an automatized scanning videomicroscope system.
Cell motility on a two-dimensional substrate was analyzed by following a large
population of cells in each culture.
Average velocities, their distribution within the population and persistence of
migration were calculated from the cell trajectories.
Results. Irradiation increases both the persistence of migration and
the heterogeneity of the cell population.
Moreover, it results in a non-monotonous alteration of cell motility: While >
10 Gy doses impair motion, exposure to 2 Gy increases velocities by 20%.
Taxol treatment reduced the motility of irradiated cells, while slightly
increased the velocities of non-irradiated cells.
We thus show that - at least for certain glioblastoma cells - both irradiation
and Taxol treatment can substantially and synergistically influence cell
motility.
Conclusions. High grade gliomas are characterized by bad prognosis and
poor response to therapy.
The unexpected motogenic effect of low-dose radiation and paclitaxel treatments
highlight the importance of similar investigations to develop more effective
clinical treatments.
PMID: 15072462 [PubMed]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15072462
|
