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Association between laminin-8 and glial tumor grade,
recurrence, and patient survival
Ljubimova JY, Fugita M, Khazenzon NM, Das A, Pikul BB, Newman D, Sekiguchi K,
Sorokin LM, Sasaki T, Black KL
Department of Surgery, Maxine Dunitz Neurosurgical Institute, Cedars-Sinai
Medical Center, Los Angeles, California 90048, USA. ljubimovaj@cshs.org
Background. The authors previously sought to identify novel markers of glioma
invasion and recurrence.
Their research demonstrated that brain gliomas
overexpressed a subset of vascular basement components, laminins, that contained
the alpha4 chain.
One of these laminins, laminin-8, was found to be present in
highly invasive and malignant glioblastoma multiforme (GBM) (Grade 4
astrocytoma); its expression was associated with a decreased time to tumor
recurrence, and it was found in vitro to promote invasion of GBM cell lines.
Methods. In the current study, the authors studied glial tumors of different
grades in an attempt to correlate laminin-8 expression with tumor recurrence and
patient survival.
Immunohistochemistry and Western blot analysis were used to
detect laminin isoforms of interest.
Results. Using immunohistochemistry and
Western blot analysis, the authors confirmed high levels of laminin-8 expression
in approximately 75% of the GBM cases examined and in their adjacent tissues,
whereas astrocytomas of lower grades expressed for the most part a different
isoform, laminin-9, which also was found in low amounts in normal brain tissue
and benign meningiomas.
Overexpression of laminin-8 in GBM was found to be
associated with a statistically significant shorter time to tumor recurrence (P
< 0.0002) and a decreased patient survival time (P < 0.015).
Conclusions.
The data suggest that laminin-8, which may facilitate tumor invasion,
contributes to tumor regrowth after therapy.
Laminin-8 may be used as a
predictor of tumor recurrence and patient survival and as a potential molecular
target for glioma therapy.
PMID: 15274074 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15274074
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