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In vivo growth suppression of rat C6 glioma transplanted
in rat brain using antisense oligonucleotide for microtubule-associated
protein 1A messenger ribonucleic acid
Matsuno A, Katayama H, Murakami M, Nagashima T
Department of Neurosurgery, Teikyo University Ichihara Hospital, Ichihara
City, Chiba, Japan. akirakun@med.teikyo-u.ac.jp
Microtubule-associated proteins (MAPs) are essential for various cellular
processes such as mitosis.
The aim of this study was to verify that the
suppression of MAP 1A using antisense oligonucleotide can suppress the in
vivo proliferation of C6 glioma cells transplanted in rat brain.
After 7
days from transplantation, antisense, sense or scramble phosphorothioate
oligodeoxynucleotide for MAP 1A mRNA was gradually delivered into the
established tumours through amini-osmotic pump.
The mean diameters of the
tumours from rats treated with antisense, sense and scramble
phosphorothioate oligodeoxynucleotide for MAP 1A mRNA were 2.33, 4.625 and
5.25 mm.
There was statistically significant difference in diameters of
tumours between rats treated with antisense oligodeoxynucleotide, and those
treated with sense or scramble oligodeoxynucleotide.
This study strongly
suggests the important role of MAP 1A in cell proliferation and its
suppressionmay serve as a novel antitumour therapy for gliomas.
PMID: 15702832 [PubMed - in process]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15702832
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