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Interstitial Infusion of IL13-PE38QQR
in the Rat Brain Stem
Mark M. Souweidane, Giuseppe Occhiogrosso, Erika B.
Mark, Mark A. Edgar
Department of Neurological Surgery
(M.M.S., G.O., E.B.M.), Department of Surgical Pathology (M.A.D.),Weill Medical
College of Cornell University and Memorial Sloan-Kettering Cancer Center, New
York, USA
Interstitial infusion of IL13-PE38QQR, a tumor specific, chimeric
cytotoxin, into the rat brain stem was performed in an effort to assess
safety.
Six rats underwent stereotaxic cannula placement into the pontine segment of the
brain stem followed by a 24-h infusion of IL13-PE38QQR (volume of infusion (Vi)
200 μl) at a concentration of 10
μg/ml.
The animals were assessed neurologically and then sacrificed either immediately
or after 2 weeks.
All animals tolerated the infusions without exhibiting any neurological
changes.
Postmortem examination of the brains revealed no significant histological
changes beyond the site of the cannula tract.
These findings indicate that supratherapeutic concentrations of IL13-PE38QQR
administered by interstitial infusion into the rat brain stem is well tolerated
and may serve as a potential therapeutic strategy for children with diffuse
pontine gliomas.
Keywords: brain stem glioma, convection-enhanced delivery, IL-13 cytotoxin,
interstitial infusion, pontine glioma
Copyright
©
2004 Kluwer Academic Publishers. All rights reserved
Source: http://journals.kluweronline.com/article.asp?PIPS=5266135
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