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Transduction
of human glial and neuronal tumor cells with different lentivirus
vector pseudotypes
Sabine Steffens, Jessica
Tebbets, Christof M. Kramm, Dirk Lindemann, Alan Flake, Miguel Sena-Esteves
Surgery Department, Abramson
Research Center, The Children’s Hospital of Philadelphia, Philadelphia, PAUSA [S.S., J.T., A.F.,
M.S.-E.*]. Department of Pediatric
Oncology, University Children’s Hospital, Heinrich-Heine-University, Hematology, and Immunology,
Duesseldorf, Germany [C.M.K.]. Institut für Virologie, Technische Universität Dresden, Dresden, Germany [D.L.]. *Corresponding Author
Lentiviral vectors have proven
to be valuable tools for in
vitro
and in vivo
gene delivery because they can transduce dividing and non-dividing
cells efficiently, and mediate long-term gene expression.
Pseudotyping of lentiviral vectors with envelope proteins other than
VSV-G has resulted in enhanced transduction of certain cell types and
tissues.
In order to improve lentiviral vector-based gene therapy for
peripheral neuroectodermal and brain tumors, we compared the
efficiency of eight different lentivirus pseudotypes in transducing
neuronal and glial tumor cell lines.
Here, lentiviral vectors pseudotyped with the envelopes from human
foamy virus, rabies, Mokola or amphotropic murine leukemia virus
displayed the highest transduction efficiency in neuroblastomas,
whereas pseudotyping with the lymphocytic choriomeningitis virus
glycoprotein from strain Armstrong 53b resulted in the highest
transduction efficiency in gliomas.
Keywords: 10A1, gene therapy, glial,
human foamy virus, LCMV, lentivirus, Mokola, murine leukemia virus,
neuronal, pseudotyping, rabies, VSV-G
Copyright © 2004 Kluwer Academic Publishers.
All rights reserved
Source:
http://ipsapp009.kluweronline.com/IPS/content/ext/x/J/5042/I/126/A/2/abstract.htm
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