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Detection of oncogene amplifications in
medulloblastomas by comparative genomic hybridization and array-based
comparative genomic hybridization
Tong CY, Hui AB, Yin XL, Pang JC, Zhu XL, Poon WS, Ng HK
Department of Anatomical and Cellular Pathology, Prince of Wales
Hospital, The Chinese University of Hong Kong, Shatin, China.
Object. Few studies have been conducted to investigate the genomic survey
of oncogene amplification in medulloblastoma.
Low frequency of N-myc, C-myc, and epidermal grow factor receptor (EGFR) gene
amplification (< 10%) has been reported in medulloblastoma.
Previous comparative genomic hybridization (CGH) study of primary
medulloblastomas has revealed chromosomal amplification on 2p21, 3p, 5p15.3, 7q,
8q24, 11q22.3, and 17q.
The aim of this study was to detect common oncogenes involved in medulloblastoma
tumorigenesis.
Methods. The authors studied a series of 14 samples by performing CGH
and array-based CGH.
The CGH analysis detected nonrandom losses on 8p, 17p, 16q, 8q, and 1p, whereas
gains were found on 17q, 12q, 7q, and 1p.
Array-based CGH was conducted to investigate amplification of 58 oncogenes
throughout the genome of these samples.
Gene amplifications identified for the first time included PGY1 at 7q21.1, MDM2
at 12q14.3-q15, and ERBB2 at 17q21.2.
The highest frequencies of oncogene gain were detected in D17S1670 (61.5%),
PIK3CA (46.2%), PGY1 (38.5%), MET (38.5%), ERBB2 (38.5%), and CSE1L
(38.5%).
The gain in gene copy numbers was confirmed in 34 additional archival
medulloblastoma cases by using fluorescence in situ hybridization
analysis.
Conclusions. This is the first genome-wide survey of multiple oncogene
amplifications involved in the development of medulloblastoma.
Gains of several candidate oncogenes such as D17S1670, ERBB2, PIK3CA, PGY1, MET,
and CSE1L were frequently detected.
These genes may be used as molecular markers and therapeutic targets of
medulloblastomas.
PMID: 14758948 [PubMed - indexed for MEDLINE]
Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14758948
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