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Specific intensity
imaging for glioblastoma and neural cell cultures with
5-aminolevulinic acid-derived protoporphyrin IX
Frank Duffner, Rainer Ritz,
Dirk Freudenstein, Michael Weller, Klaus Dietz and
Johannes Wessels
Department
of Neurosurgery, Eberhard-Karls University of Tuebingen, Germany
[F.D., R.R., D.F.]. Department of Neurology, Eberhard-Karls University
of Tuebingen, Germany [M.W.]. Department of Medical Biometry,
Eberhard-Karls University of Tuebingen, Germany [K.D.]. Department of
Pediatrics, Eberhard-Karls University of Tuebingen, Germany [J.W.].
Department of Neurosurgery, University Hospital Tuebingen,
Hoppe-Seyler-Str. 3, 72076 Tuebingen, Germany [R.R.].
Correspondence to: Rainer Ritz, Email:
rainer.ritz@med.uni-tuebingen.de, Phone: +49-7071-2980325, Fax:
+49-7071-295245.
The fluorescence of protophorphyrin IX
(PpIX) synthesized after incubation with 5-aminolevulinic acid (5-Ala)
is used for the intraoperative visualisation of glioma cells in
vivo.
Such fluorescence may also be useful for the photodynamic therapy
(PTD) of gliomas.
A significant difference of fluorescence intensity in tumor cells
compared to neurons is required for this application.
To explore this, eight human glioma cell lines (LN-18, LN-428, U87MG,
U373MG, D247MG, U251MG, LN-308, T98G) were compared with human
astrocytes (SV-FHAS) and rat neurons after incubation for different
periods of time in vitro with 5-Ala (1mg/ml).
Fluorescence intensity profiles were measured by a digital camera
comparing glioma cell lines with control cells.
All glioma cell lines could be discriminated from neural cells by
their intensity of fluorescence by post-hoc tests for pairwise
comparisons using Tukey's honestly significant difference test, at the
global significance level of 5%.
The glioma cell lines showed significant variation in this possibly
limiting clinical use of fluorescence as a guide for resection.
Keywords: 5-aminolevulinic
acid - fluorescence - glioblastoma - photodynamic
therapy - protoporphyrin IX
© Springer 2005
Source: http://springerlink.metapress.com/openurl.asp?genre=article&eissn=1573-7373&volume=71&issue=2&spage=107
DOI: http://dx.doi.org/10.1007/s11060-004-9603-2
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