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Cellular DNA content parameters as
prognostic indicators in human astrocytomas
Basil F. El-Rayes, Camille S. Norton,
Wael Sakr, Zosia Maciorowski, Daryn Smith, Haline Pietraszkiewicz,
Maria Del Mar Alonso and John F. Ensley
Department
of Internal Medicine, Division of Hematology and Oncology, Wayne State
University, Karmanos Cancer Institute, MI, USA [B.F.E.-R., C.S.N.,
D.S., H.P., M.D.M.A., J.F.E.]. Department of Pathology, School of
Medicine, Wayne State University, Karmanos Cancer Institute, Detroit,
MI, USA [W.S.]. Curie Institute, Paris, France [Z.M.]. Harper
Hospital, 3990 John R, Detroit, MI 48201, USA [J.F.E.].
Correspondence to: John F. Ensley, Email:
ensleyj@karmanos.org, Phone: +1-313-745-9168, Fax: +1-313-993 0559.
Objective. Clinical
parameters such as grade, size and/or location of the tumor are good
predictors of outcome in patients with astrocytoma.
The objective of this study was to determine whether DNA content
parameters have a prognostic significance for this group of tumors.
Methods. Following
optimization and validation of methodology for evaluating cellular DNA
content parameters (CDCP), tumor DNA ploidy and percent S phase
fraction (SPF) were determined from 64 patients using formalin fixed,
paraffin embedded specimens (mean coefficient of variation = 4.94)
obtained over a 10-year period.
Median survival times correlated with grade (I/II =1154 vs. III/IV=
483days, P=0.0317).
Fifty-five percent of the specimens contained DNA aneuploid (DNA-A)
components (average SPF=18.3%) and 45% were DNA diploid (DNA-D)
(average SPF=9.6%).
Survival did not correlate with overall differences in DNA ploidy
(DNA-D=181 vs. DNA-A=206days, P=0.6314) when treated and
untreated tumors were analyzed.
However, a trend for prolonged median survival was observed in
patients whose tumors were untreated with respect to cytotoxic therapy
based on DNA ploidy status (DNA-D=275 vs. DNA-A=15days, P=0.3408).
Survival for all patients did not correlate with median SPF (<
13.5% av.= 121 vs. > 13.5% av.= 154days, P=0.6534).
Conclusion.
DNA content parameters may correlate with the natural history and
treatment outcome of newly diagnosed untreated patients with
astrocytomas.
Keywords: aneuploid - astrocytoma - diploid
© Springer 2005
Source: http://springerlink.metapress.com/openurl.asp?genre=article&eissn=1573-7373&volume=71&issue=2&spage=85
DOI: http://dx.doi.org/10.1007/s11060-004-6044-x
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