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Ganglioside GM3 inhibits proliferation
and invasion of glioma
Yasunori Fujimoto, Shuichi Izumoto,
Tsuyoshi Suzuki, Manabu Kinoshita, Naoki Kagawa,
Kouichi Wada, Naoya Hashimoto, Motohiko Maruno, Yuji Nakatsuji
and Toshiki Yoshimine
Department
of Neurosurgery, Osaka University Medical School, Suita, Osaka, Japan
[Y.F., S.I., T.S., M.K., N.K. K.W. N.H., M.M., T.Y.]. Department of
Neurology, Osaka University Medical School, Suita,Osaka, Japan [Y.N.].
Department of Neurosurgery, Osaka University Medical School, E6 2-2
Yamadaoka, Suita, Osaka 565-0871, Japan [S.I.].
Correspondence to: Shuichi Izumoto, Email:
sizumoto@nsurg.med.osaka-u.ac.jp, Phone: +81-6-6879-3652, Fax:
+81-6-6879-3659
GM3, the simplest ganglioside,
modulates cell adhesion, proliferation and differentiation in the
central nervous system and exogenously added GM3 regulates cell–cell
and cell–extracellular matrix adhesion and induces apoptosis.
To assess the anti-tumor action of
exogenous GM3, we examined its effect on the proliferation and
invasion of glioma cells.
Its inhibitory effect on cell
proliferation was demonstrated in vitro by
3-(4,5-dimethyl-2-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT)
assay and in vivo in rats with meningeal gliomatosis whose
survival was significantly prolonged by the intrathecal injection of
GM3.
Terminal deoxynucleotidyl
transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay
revealed that GM3 induced glioma cell apoptosis in vitro and in
vivo. In rat brain slice cultures, GM3 suppressed the invasion of
glioma cells; this effect manifested earlier than the inhibition of
cell proliferation and before apoptosis induction.
Our results suggest exogenous GM3 as a
potential therapeutic agent in patients with glioma requiring adjuvant
therapy.
Keywords apoptosis - brain
slice culture - ganglioside - glioma - GM3 - invasion
© Springer 2005
Source: http://www.springerlink.com/openurl.asp?genre=article&eissn=1573-7373&volume=71&issue=2&spage=99
DOI: http://dx.doi.org/10.1007/s11060-004-9602-3
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