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Tamoxifen-induced cell death and expression of neurotrophic factors in
cultured C6 glioma cells Yong-Jung Kim, Chang-Joong Lee,
Uhn Lee and Young-Mi Yoo
Department of Biological Sciences,
College of Natural Sciences, Inha University, South Korea [Y.-J.K.,
C.-J.L.]. Department of Neurosurgery,Gachon Medical College, Gil
Medical Center, Incheon, South Korea [U.L., Y.-M.Y.]. Neuroscience
Institute, Department of Neurosurgery, Gachon Medical School, Gil
Medical Center, 1198 Guwol-dong, Namdong-Gu, Incheon, 405-760, Korea
[Y.-M.Y.].
Correspondence to: Young-Mi Yoo,
Email: neurodragon@naver.com, Phone: +82-32-460-3090, Fax: +82-32-460-3899.
The effects of ( Z)-2[ p
-(1,2-diphenyl-1-butenyl)phenoxy]-N ,N -dimethylamine
citrate (tamoxifen) on cell survival and the expression of
neurotrophic factors (NTF) were investigated in rat C6 glioma cells
(C6).
C6 cells do not express the estrogen receptor.
Cytotoxic effect
was detected from 24 h after the treatment with 10 μM
tamoxifen and increased with time in a dose-dependent manner.
C6 cells
treated with tamoxifen also displayed various morphological types such
as elliptical, round and aggregated form.
As the treatment time
increased, the proliferation of C6 cells was reduced remarkably and
most of them became the round or aggregated form.
To examine the relationship of the expression of NTF and the
cytotoxicity of tamoxifen, the mRNA level of brain-derived
neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF),
and basic fibroblast growth factor (bFGF) was measured after 24 h
treatment with tamoxifen by RT-PCR.
The expression of mRNA of BDNF or
GDNF in C6 cells treated with various concentrations of tamoxifen was
comparable to controls.
The expression of bFGF mRNA was significantly
reduced in C6 cells treated with 10 or 15 μM
tamoxifen.
The results suggest that tamoxifen exerts cytotoxic effect
on estrogen receptor-negative C6 cells through the inhibition of the
transcription of bFGF.
Keywords: BDNF - bFGF - C6
glioma - GDNF - tamoxifen
© Springer 2005
Source: http://springerlink.metapress.com/openurl.asp?genre=article&eissn=1573-7373&volume=71&issue=2&spage=121
DOI: http://dx.doi.org/10.1007/s11060-004-0984-z
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