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Expression of Cathepsin B and
microvascular density increases with higher grade of astrocytomas
Maode Wang, Jianjian Tang,
Shouxun Liu, Daizo Yoshida and Akira Teramoto
Department
of Neurosurgery, The First Hospital, Xi an
Jiaotong University, 710061, No. 1 Jiankang Road, Xian,
China [M.W., J.T., S.L.]; Department of Neurosurgery, Nippon Medical
School, Bunkyo-ku, Tokyo, Japan [D.Y., A.T.].
Correspondence to: Maode Wang, Email: maodewang@yahoo.com.cn, Phone:
+86-29-526-8717, Fax: +86-29-523-8806.
Samples were taken from supratentorial
gliomas border and normal brain autopsy which were divided into four
groups, these including eight cases normal brain tissues, 30 cases of
astrocytomas, 25 cases of anaplastic astrocytomas and 22 cases of
glioblastomas.
Cathepin B (CB) expression and microvessel
density (MVD) were determined with immunohistochemical studies.
Staining results of CB was scored
according to the percentage of positive cells, graded as negative
(–), weak (+), moderate (++), and strong (+ + +).
MVD was analyzed by Weidner s
revised technique.
CB positive staining was negative in eight
cases of normal brain tissue.
Only 9 out of 30 cases of astrocytomas
showed a low percentage of positive cells that were stained in a
light, diffuse cytoplasmic pattern (score +).
Twenty-two out of 35 cases of anaplastic
astrocytomas showed positive light, granular staining pattern, it
including five samples (score +), and 17 samples (score + +).
In contrast, all 22 cases of glioblastomas
were stained all, and it was present in a course, granular staining
pattern with an intensity of score (+ +) of two sample, and score (+ +
+) of 20 samples.
Positive staining tumor cells were found
in extracellular matrix (ECM), basement membrane (BM), and the
endothelial cells of blood vessels were also positive stained.
Along with elevating glioma grade, CB
expression and MVD value were both increased.
Therefore, it showed MVD value was
positive correlated with expression of CB.
It highly suggested that CB and
angiogenesis plays an important role in glioma progression.
Keywords: cathepsin
B - gliomas - invasion - microvessel
density
© Springer 2005
Source: http://springerlink.metapress.com/openurl.asp?genre=article&eissn=1573-7373&volume=71&issue=1&spage=3
DOI: http://dx.doi.org/10.1007/s11060-004-9163-5
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