From: Subject: Oncogene - Abstract of article: CXCR4 expression mediates glioma cell invasiveness Date: Tue, 16 May 2006 19:04:28 +0200 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0000_01C6791B.8E2E6180" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869 This is a multi-part message in MIME format. ------=_NextPart_000_0000_01C6791B.8E2E6180 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.nature.com/onc/journal/v25/n19/abs/1209302a.html;jsessionid=B449B61B46A38B8E8973BB1C88AD233E Oncogene - Abstract = of article: CXCR4 expression mediates glioma cell = invasiveness
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Short Communication

Oncogene (2006) 25, 2801=962806.=20 doi:10.1038/sj.onc.1209302; published online 9 January 2006

CXCR4 expression mediates glioma cell invasiveness

M Ehtesham1,2,3,=20 J A Winston1,=20 P Kabos4=20 and R C Thompson1,3

  1. 1Department of Neurological Surgery, = Vanderbilt=20 University Medical Center, Nashville, TN, USA
  2. 2Department of Cancer Biology, Vanderbilt = University=20 Medical Center, Nashville, TN, USA
  3. 3Vanderbilt Ingram Cancer Center, Vanderbilt = University=20 Medical Center, Nashville, TN, USA
  4. 4Department of Medicine, University of = Southern=20 California, Los Angeles, CA, USA

Correspondence: Dr M Ehtesham, T-4224, Medical Center = North,=20 Vanderbilt University Medical Center, Nashville, TN 37232-2380, USA. = E-mail: moneeb.ehtesham@vanderbilt= .edu

Received 18 December 2004; Revised=20 27 October 2005; Accepted 4 November 2005; Published = online=20 9 January 2006.

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Abstract

Glioblastoma multiforme is a highly invasive tumor = bearing a=20 dismal prognosis. Experimental strategies that focus on the specific = biological=20 cues governing the invasive capacity of these tumors may hold = significant=20 therapeutic promise. In this context, we describe the in vitro = and in=20 vivo association of the cell surface chemokine receptor, CXCR4, with = the=20 development of an invasive phenotype in malignant glioblastoma. We = demonstrate=20 that invasive populations of glioma cells overexpress CXCR4 at the = message and=20 protein levels, and that this expression ranges from 25- to 89-fold = higher than=20 that found in noninvasive tumor cells. Furthermore, neutralization of = CXCR4=20 significantly impairs the in vitro invasive capacity of malignant = glial=20 cells. In addition, glioma cells secrete CXCL12 and demonstrate robust = invasive=20 capacity toward a CXCL12 gradient in vitro. These findings = underscore the=20 importance of CXCR4 as a potential therapeutic target for the treatment = of=20 invasive glioblastoma.

Keywords:

chemokine, CXCR4, invasion, glioma, brain=20 tumor

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Oncogene

ISSN: 0950-9232

EISSN: 1476-5594

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