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Etiology and Pathogenesis
> Cancer
Stem Cells
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Nature Biotechnology, Advance online publication, Published
online: 26 February 2006
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Abstract |
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CD30 is a survival factor and a
biomarker for transformed human pluripotent stem cells
Daniella
Herszfeld1, 5, Ernst
Wolvetang1, 5, Emma
Langton-Bunker1, Tung-Liang
Chung1, Adam A Filipczyk1,
Souheir Houssami1, Pegah Jamshidi1,
Karen Koh1, Andrew L Laslett1,
Anna Michalska1, Linh Nguyen1,
Benjamin E Reubinoff1, 4, Irene
Tellis1, Jonathan M Auerbach2,
Carol J Ording2, Leendert H J
Looijenga3 & Martin F
Pera1
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1Monash
Institute of Medical Research, Monash University, and the Australian Stem
Cell Centre, Bldg. 75 STRIP, Wellington Road, Clayton, Victoria 3800
Australia. 2Stem Cell Center, American Type
Culture Collection, 10801 University Boulevard, Manassas, Virginia 20110,
USA. 3Pathology/Lab. Exp. Patho-Oncology,
Erasmus MC–University Medical Center Rotterdam, Daniel den Hoed Cancer
Center, Josephine Nefkens Institute, Building Be, Room 430b, P.O. Box 1738,
3000 DR Rotterdam, The Netherlands. 4Present
address: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne
Savad Institute of Gene Therapy and The Department of Gynecology, Hadassah
University Hospital, Ein-Kerem, P.O.B. 12000, Jerusalem 91120, Israel. 5These
authors contributed equally to this work. Correspondence
should be addressed to Martin F Pera martin.pera@med.monash.edu.au.
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The
application of human embryonic stem (hES) cells in regenerative
medicine will require rigorous quality control measures to ensure the
safety of hES cell–derived grafts.
During
propagation in vitro, hES cells can acquire
cytogenetic abnormalities as well as submicroscopic genetic lesions,
such as small amplifications or deletions.
Many
of the genetic abnormalities that arise in hES cell cultures are also
implicated in human cancer development.
The
causes of genetic instability of hES cells in culture are poorly
understood, and commonly used cytogenetic methods for detection of
abnormal cells are capable only of low-throughput analysis on small
numbers of cells.
The
identification of biomarkers of genetic instability in hES cells would
greatly facilitate the development of culture methods that preserve
genomic integrity.
Here
we show that CD30, a member of the tumor necrosis factor receptor
superfamily, is expressed on transformed but not normal hES cells, and
that CD30 expression protects hES cells against apoptosis.
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©2006 Nature Publishing Group
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DOI: http://dx.doi.org/10.1038/nbt1197
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Source: http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1197.html
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