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Prognostic
value of increase in transcript levels of Tp73 ΔEx2-3 isoforms in
low-grade glioma patients
M Wager1, J
Guilhot2, J-L Blanc1, S Ferrand1,
S Milin3, B Bataille1, F
Lapierre1, S Denis4, T Chantereau4,
C-J Larsen4 and L Karayan-Tapon4
1Neurosurgery
Department, University Hospital, Poitiers, France. 2Clinical
Research Centre, University Hospital, Poitiers, France. 3Department
of Pathology, University Hospital, Poitiers, France. 4Laboratory
of Molecular Oncology, University Hospital, Poitiers, France. --
Correspondence to: Dr M Wager, Service de Neurochirurgie, H3A Tour
Jean Bernard, 350 Avenue Jacques Cœur, CHU La Miletrie, Poitiers
Cedex 86021, France. E-mail: m.wager@chu-poitiers.fr. -- Received 24
March 2006; revised 21 August 2006; accepted 21 August 2006.
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Glial tumours are a
devastating, poorly understood condition carrying a gloomy prognosis
for which clinicians sorely lack reliable predictive parameters
facilitating a sound treatment strategy.
Tp73, a p53 family
member, expresses two main classes of isoforms - transactivatory
activity (TA)p73 and ΔTAp73
- exhibiting tumour suppressor gene and oncogene properties,
respectively.
The authors
examined their expression status in high- and low-grade adult gliomas.
Isoform-specific
real-time reverse transcription-polymerase chain reaction was used for
the analysis of Tp73 isoform transcript expression in a series of 51
adult patients harbouring glial tumours, in order to compare tumour
grades with each other, and with non-tumoural samples obtained from
epileptic patients as well.
Our data
demonstrate increase of TAp73 and ΔTAp73
transcript levels at onset and early stage of the disease.
We also show that ΔEx2-3
isoform expression in low-grade tumours anticipates clinical and
imaging progression to higher grades, and correlates to the patients'
survival.
Expression levels
of P1 promoter generated Tp73 isoforms - and particularly ΔEx2-3
- indeed allow for prediction of the clinical progression of low-grade
gliomas in adults.
Our data are the
first such molecular biology report regarding low-grade tumours and as
such should be of help for sound decision-making.
Keywords:
Tp73 isoforms; low-grade gliomas; prognosis; quantitative
reverse-transcription polymerase chain reaction (QRT-PCR)
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