From: Subject: Cell Research - Abstract of article: Differentiation profile of brain tumor stem cells: a comparative study with neural stem cells Date: Fri, 22 Feb 2008 08:48:41 +0100 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0041_01C8752F.B969A3C0" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0041_01C8752F.B969A3C0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.nature.com/cr/journal/v16/n12/abs/7310104a.html;jsessionid=A9A1674B573A716DEC46CE8611C5F9C9 Cell Research - = Abstract of article: Differentiation profile of brain tumor stem cells: = a comparative study with neural stem cells

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Original Article

Cell Research (2006) 16: 909=96915.=20 doi:10.1038/sj.cr.7310104; published online 7 November 2006

Differentiation profile of brain tumor stem cells: a = comparative=20 study with neural stem cells

Quan Bin Zhang¹,=20 Xiao Yan Ji¹,=20 Qiang Huang¹,=20 Jun Dong¹,=20 Yu De Zhu¹=20 and Qing Lan¹

¹Neurosurgical Department and = Brain Tumor=20 Research Laboratory, Second Affiliated Hospital of Suzhou University, = Suzhou=20 215004, China

Correspondence: Qiang Huang, Tel: +86-512-67783937; = E-mail: szqhdnah@pub.sz.jsinfo.net=

Received 18 May 2006; Revised=20 23 August 2006; Accepted 19 September 2006. =

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Abstract

Understanding of the differentiation profile of = brain tumor=20 stem cells (BTSCs), the key ones among tumor cell population, through = comparison=20 with neural stem cells (NSCs) would lend insight into the origin of = glioma and=20 ultimately yield new approaches to fight this intractable disease. Here, = we=20 cultured and purified BTSCs from surgical glioma specimens and NSCs from = human=20 fetal brain tissue, and further analyzed their cellular biological = behaviors,=20 especially their differentiation property. As expected, NSCs = differentiated into=20 mature neural phenotypes. In the same differentiation condition, = however, BTSCs=20 exhibited distinguished differences. Morphologically, cells grew = flattened and=20 attached for the first week, but gradually aggregated and reformed = floating=20 tumor sphere thereafter. During the corresponding period, the expression = rate of=20 undifferentiated cell marker CD133 and nestin in BTSCs kept decreasing, = but 1=20 week later, they regained ascending tendency. Interestingly, the = differentiated=20 cell markers GFAP and -tubu= linIII=20 showed an expression change inverse to that of undifferentiated cell = markers.=20 Taken together, BTSCs were revealed to possess a capacity to resist=20 differentiation, which actually represents the malignant behaviors of=20 glioma.

Keywords:

brain tumor stem cell, neural stem cell,=20 differentiation

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Cell Research

ISSN: 1001-0602

EISSN: 1748-7838

=A9 2008 Shanghai Institutes = for=20 Biological Sciences

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