From: Subject: BioMed Central | Abstract | 1471-2407-7-233 | Correlation between the progressive cytoplasmic expression of a novel small heat shock protein (Hsp16.2) and malignancy in brain tumors Date: Fri, 25 Jan 2008 11:10:01 +0100 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0000_01C85F42.D48D9D30" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0000_01C85F42.D48D9D30 Content-Type: text/html; charset="utf-8" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.biomedcentral.com/1471-2407/7/233 =EF=BB=BF BioMed Central | = Abstract | 1471-2407-7-233 | Correlation between the progressive = cytoplasmic expression of a novel small heat shock protein (Hsp16.2) and = malignancy in brain tumors
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Research article

Correlation between the progressive cytoplasmic expression of = a novel=20 small heat shock protein (Hsp16.2) and malignancy in brain tumors =

Eva Pozsgai ,=20 Eva Gomori ,=20 Andras Szigeti ,=20 Arpad Boronkai ,=20 Ferenc Gallyas Jr. ,=20 Balazs Sumegi and = Szabolcs Bellyei

<= BR>

BMC Cancer 2007, = 7:233doi:10.1186/1471-2407-7-233

=
Published:21 December 2007
<= A=20 name=3Dabstract>

Abstract (provisional)

Background

Small heat shock proteins are molecular chaperones that protect = proteins against stress-induced aggregation. They have also been = found to=20 have anti-apoptotic activity and to play a part in the development = of=20 tumors. Recently, we identified a new small heat shock protein, = Hsp16.2=20 which displayed increased expression in neuroectodermal tumors. = Our aim=20 was to investigate the expression of Hsp16.2 in different types of = brain=20 tumors and to correlate its expression with the histological grade = of the=20 tumor.

Methods

Immunohistochemistry with a polyclonal antibody to Hsp16.2 was = carried=20 out on formalin-fixed, paraffin-wax-embedded sections using the=20 streptavidin-biotin method. 91 samples were examined and their=20 histological grade was defined. According to the intensity of = Hsp16.2=20 immunoreactivity, low (+), moderate (++), high (+++) or none (-) = scores=20 were given. Immunoblotting was carried out on 30 samples of brain = tumors=20 using SDS-polyacrylamide gel electrophoresis and = Western-blotting.

Results

Low grade (grades 1-2) brain tumors displayed low cytoplasmic = Hsp16.2=20 immunoreactivity, grade 3 tumors showed moderate cytoplasmic = staining,=20 while high grade (grade 4) tumors exhibited intensive cytoplasmic = Hsp16.2=20 staining. Immunoblotting supported the above mentioned results. = Normal=20 brain tissue acted as a negative control for the experiment, since = the=20 cytoplasm did not stain for Hsp16.2. There was a positive = correlation=20 between the level of Hsp16.2 expression and the level of anaplasia = in=20 different malignant tissue samples.

Conclusion

Hsp16.2 expression was directly correlated with the = histological grade=20 of brain tumors, therefore Hsp16.2 may have relevance as becoming = a=20 possible tumor marker.


The complete article is available as a = pro= visional=20 PDF. The fully formatted PDF and HTML versions are in = production.=20

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_u5_ugifpath=3D"/__utm.gif"; // set the web path to the __utm.gif = file var _u5_utsp=3D"|"; // transaction field separator var _u5_uflash=3D1; // set flash version detect option = (1=3Don|0=3Doff) var _u5_utitle=3D1; // set the document title detect option = (1=3Don|0=3Doff) //-- UTM Campaign Tracking Settings var _u5_uctm=3D0; // set campaign tracking module (1=3Don|0=3Doff) var _u5_ucto=3D"15768000"; // set timeout in seconds (6 month default) var _u5_uccn=3D"utm_campaign"; // name var _u5_ucmd=3D"utm_medium"; // medium (cpc|cpm|link|email|organic) var _u5_ucsr=3D"utm_source"; // source var _u5_uctr=3D"utm_term"; // term/keyword var _u5_ucct=3D"utm_content"; // content var _u5_ucid=3D"utm_id"; // id number var _u5_ucno=3D"utm_nooverride"; // don't override //-- Auto/Organic Sources and Keywords var _u5_uOsr=3Dnew Array(); var _u5_uOkw=3Dnew Array(); _u5_uOsr[0]=3D"google"; _u5_uOkw[0]=3D"q"; _u5_uOsr[1]=3D"yahoo"; _u5_uOkw[1]=3D"p"; _u5_uOsr[2]=3D"msn"; _u5_uOkw[2]=3D"q"; _u5_uOsr[3]=3D"aol"; _u5_uOkw[3]=3D"query"; _u5_uOsr[4]=3D"lycos"; _u5_uOkw[4]=3D"query"; _u5_uOsr[5]=3D"ask"; _u5_uOkw[5]=3D"q"; _u5_uOsr[6]=3D"altavista"; _u5_uOkw[6]=3D"q"; _u5_uOsr[7]=3D"search"; _u5_uOkw[7]=3D"q"; _u5_uOsr[8]=3D"netscape"; _u5_uOkw[8]=3D"query"; _u5_uOsr[9]=3D"earthlink"; _u5_uOkw[9]=3D"q"; _u5_uOsr[10]=3D"cnn"; _u5_uOkw[10]=3D"query"; _u5_uOsr[11]=3D"looksmart"; _u5_uOkw[11]=3D"key"; _u5_uOsr[12]=3D"about"; _u5_uOkw[12]=3D"terms"; _u5_uOsr[13]=3D"excite"; _u5_uOkw[13]=3D"qkw"; _u5_uOsr[14]=3D"mamma"; _u5_uOkw[14]=3D"query"; _u5_uOsr[15]=3D"alltheweb"; _u5_uOkw[15]=3D"q"; _u5_uOsr[16]=3D"gigablast"; _u5_uOkw[16]=3D"q"; _u5_uOsr[17]=3D"voila"; _u5_uOkw[17]=3D"kw"; _u5_uOsr[18]=3D"virgilio"; _u5_uOkw[18]=3D"qs"; _u5_uOsr[19]=3D"teoma"; _u5_uOkw[19]=3D"q"; //-- Auto/Organic Keywords to Ignore var _u5_uOno=3Dnew Array(); //_u5_uOno[0]=3D"urchin"; //_u5_uOno[1]=3D"urchin.com"; //_u5_uOno[2]=3D"www.urchin.com"; //-- Referral domains to Ignore var _u5_uRno=3Dnew Array(); //_u5_uRno[0]=3D".urchin.com"; //-- **** Don't modify below this point *** var = _u5_uff,_u5_udh,_u5_udt,_u5_udo=3D"",_u5_uu,_u5_ufns=3D0,_u5_uns=3D0,_u5_= ur=3D"-",_u5_ufno=3D0,_u5_ust=3D0,_u5_ujv=3D"-",_u5_ubd=3Ddocument,_u5_ud= l=3D_u5_ubd.location,_u5_uwv=3D"6.1"; var _u5_ugifpath2=3D"http://service.urchin.com/__utm.gif"; if (_u5_udl.protocol=3D=3D"https:") = _u5_ugifpath2=3D"https://service.urchin.com/__utm.gif"; function u5_urchinTracker(page) { if (_u5_udl.protocol=3D=3D"file:") return; if (_u5_uff && (!page || page=3D=3D"")) return; var a,b,c,v,x=3D"",s=3D"",f=3D0; var nx=3D" expires=3DSun, 18 Jan 2038 00:00:00 GMT;"; var dc=3D_u5_ubd.cookie; _u5_udh=3D_u5_uDomain(); _u5_uu=3DMath.round(Math.random()*2147483647); _u5_udt=3Dnew Date(); _u5_ust=3DMath.round(_u5_udt.getTime()/1000); a=3Ddc.indexOf("__utma=3D"+_u5_udh); b=3Ddc.indexOf("__utmb=3D"+_u5_udh); c=3Ddc.indexOf("__utmc=3D"+_u5_udh); if (_u5_udn && _u5_udn!=3D"") { _u5_udo=3D" domain=3D"+_u5_udn+";"; } if (_u5_utimeout && _u5_utimeout!=3D"") { x=3Dnew Date(_u5_udt.getTime()+(_u5_utimeout*1000)); x=3D" expires=3D"+x.toGMTString()+";"; } s=3D_u5_udl.search; if(s && s!=3D"" && s.indexOf("__utma=3D")>=3D0) { a=3D_u5_uGC(s,"__utma=3D","&"); b=3D_u5_uGC(s,"__utmb=3D","&"); c=3D_u5_uGC(s,"__utmc=3D","&"); if (a!=3D"-" && b!=3D"-" && c!=3D"-") f=3D1; else if(a!=3D"-") f=3D2; } if(f=3D=3D1) { _u5_ubd.cookie=3D"__utma=3D"+a+"; path=3D/;"+nx; _u5_ubd.cookie=3D"__utmb=3D"+b+"; path=3D/;"+x; _u5_ubd.cookie=3D"__utmc=3D"+c+"; path=3D/;"; } else if (f=3D=3D2) { a=3D_u5_uFixA(s,"&",_u5_ust); _u5_ubd.cookie=3D"__utma=3D"+a+"; path=3D/;"+nx; _u5_ubd.cookie=3D"__utmb=3D"+_u5_udh+"; path=3D/;"+x; _u5_ubd.cookie=3D"__utmc=3D"+_u5_udh+"; path=3D/;"; _u5_ufns=3D1; } else if (a>=3D0 && b>=3D0 && c>=3D0) { _u5_ubd.cookie=3D"__utmb=3D"+_u5_udh+"; path=3D/;"+x+_u5_udo; } else { if (a>=3D0) a=3D_u5_uFixA(_u5_ubd.cookie,";",_u5_ust); else a=3D_u5_udh+"."+_u5_uu+"."+_u5_ust+"."+_u5_ust+"."+_u5_ust+".1"; _u5_ubd.cookie=3D"__utma=3D"+a+"; path=3D/;"+nx+_u5_udo; _u5_ubd.cookie=3D"__utmb=3D"+_u5_udh+"; path=3D/;"+x+_u5_udo; _u5_ubd.cookie=3D"__utmc=3D"+_u5_udh+"; path=3D/;"+_u5_udo; _u5_ufns=3D1; } if (s && s!=3D"" && s.indexOf("__utmv=3D")>=3D0) { if ((v=3D_u5_uGC(s,"__utmv=3D","&"))!=3D"-") { _u5_ubd.cookie=3D"__utmv=3D"+unescape(v)+"; path=3D/;"+nx+_u5_udo; } } _u5_uInfo(page); _u5_ufns=3D0; _u5_ufno=3D0; _u5_uff=3D1; } u5_urchinTracker(); function _u5_uInfo(page) { var p,s=3D"",pg=3D_u5_udl.pathname+_u5_udl.search; if (page && page!=3D"") pg=3Descape(page); _u5_ur=3D_u5_ubd.referrer; if (!_u5_ur || _u5_ur=3D=3D"") { _u5_ur=3D"-"; } else { p=3D_u5_ur.indexOf(_u5_ubd.domain); if ((p>=3D0) && (p<=3D8)) { _u5_ur=3D"0"; } if (_u5_ur.indexOf("[")=3D=3D0 && = _u5_ur.lastIndexOf("]")=3D=3D(_u5_ur.length-1)) { _u5_ur=3D"-"; } } s+=3D"&utmn=3D"+_u5_uu; if (_u5_ufsc) s+=3D_u5_uBInfo(page); if (_u5_uctm && (!page || page=3D=3D"")) s+=3D_u5_uCInfo(); if (_u5_utitle && _u5_ubd.title && _u5_ubd.title!=3D"") = s+=3D"&utmdt=3D"+escape(_u5_ubd.title); if (_u5_udl.hostname && _u5_udl.hostname!=3D"") = s+=3D"&utmhn=3D"+escape(_u5_udl.hostname); if (!page || page=3D=3D"") s+=3D"&utmr=3D"+_u5_ur; s+=3D"&utmp=3D"+pg; if (_u5_userv=3D=3D0 || _u5_userv=3D=3D2) { var i=3Dnew Image(1,1); i.src=3D_u5_ugifpath+"?"+"utmwv=3D"+_u5_uwv+s; i.onload=3Dfunction() {_u5_uVoid();} } if (_u5_userv=3D=3D1 || _u5_userv=3D=3D2) { var i2=3Dnew Image(1,1); = i2.src=3D_u5_ugifpath2+"?"+"utmwv=3D"+_u5_uwv+s+"&utmac=3D"+_u5_uacct+"&u= tmcc=3D"+_u5_uGCS(); i2.onload=3Dfunction() { _u5_uVoid(); } } return; } function _u5_uVoid() { return; } function _u5_uCInfo() { if (!_u5_ucto || _u5_ucto=3D=3D"") { _u5_ucto=3D"15768000"; } var c=3D"",t=3D"-",t2=3D"-",o=3D0,cs=3D0,cn=3D0;i=3D0; var s=3D_u5_udl.search; var z=3D_u5_uGC(s,"__utmz=3D","&"); var x=3Dnew Date(_u5_udt.getTime()+(_u5_ucto*1000)); var dc=3D_u5_ubd.cookie; x=3D" expires=3D"+x.toGMTString()+";"; if (z!=3D"-") { _u5_ubd.cookie=3D"__utmz=3D"+unescape(z)+"; = path=3D/;"+x+_u5_udo; return ""; } z=3Ddc.indexOf("__utmz=3D"+_u5_udh); if (z>-1) { z=3D_u5_uGC(dc,"__utmz=3D"+_u5_udh,";"); } else { z=3D"-"; } t=3D_u5_uGC(s,_u5_ucid+"=3D","&"); t2=3D_u5_uGC(s,_u5_ucsr+"=3D","&"); if ((t!=3D"-" && t!=3D"") || (t2!=3D"-" && t2!=3D"")) { if (t!=3D"-" && t!=3D"") { c+=3D"utmcid=3D"+_u5_uEC(t); if (t2!=3D"-" = && t2!=3D"") c+=3D"|utmcsr=3D"+_u5_uEC(t2); } else { if (t2!=3D"-" && t2!=3D"") c+=3D"utmcsr=3D"+_u5_uEC(t2); } t=3D_u5_uGC(s,_u5_uccn+"=3D","&"); if (t!=3D"-" && t!=3D"") c+=3D"|utmccn=3D"+_u5_uEC(t); else c+=3D"|utmccn=3D(not+set)"; t=3D_u5_uGC(s,_u5_ucmd+"=3D","&"); if (t!=3D"-" && t!=3D"") c+=3D"|utmcmd=3D"+_u5_uEC(t); else c+=3D"|utmcmd=3D(not+set)"; t=3D_u5_uGC(s,_u5_uctr+"=3D","&"); if (t!=3D"-" && t!=3D"") c+=3D"|utmctr=3D"+_u5_uEC(t); else { t=3D_u5_uOrg(1); if (t!=3D"-" && t!=3D"") = c+=3D"|utmctr=3D"+_u5_uEC(t); } t=3D_u5_uGC(s,_u5_ucct+"=3D","&"); if (t!=3D"-" && t!=3D"") c+=3D"|utmcct=3D"+_u5_uEC(t); t=3D_u5_uGC(s,_u5_ucno+"=3D","&"); if (t=3D=3D"1") o=3D1; if (z!=3D"-" && o=3D=3D1) return ""; } if (c=3D=3D"-" || c=3D=3D"") { c=3D_u5_uOrg(); if (z!=3D"-" && = _u5_ufno=3D=3D1) return ""; } if (c=3D=3D"-" || c=3D=3D"") { if (_u5_ufns=3D=3D1) c=3D_u5_uRef(); if = (z!=3D"-" && _u5_ufno=3D=3D1) return ""; } if (c=3D=3D"-" || c=3D=3D"") { if (z=3D=3D"-" && _u5_ufns=3D=3D1) { = c=3D"utmccn=3D(direct)|utmcsr=3D(direct)|utmcmd=3D(none)"; } if (c=3D=3D"-" || c=3D=3D"") return ""; } if (z!=3D"-") { i=3Dz.indexOf("."); if (i>-1) i=3Dz.indexOf(".",i+1); if (i>-1) i=3Dz.indexOf(".",i+1); if (i>-1) i=3Dz.indexOf(".",i+1); t=3Dz.substring(i+1,z.length); if (t.toLowerCase()=3D=3Dc.toLowerCase()) cs=3D1; t=3Dz.substring(0,i); if ((i=3Dt.lastIndexOf(".")) > -1) { t=3Dt.substring(i+1,t.length); cn=3D(t*1); } } if (cs=3D=3D0 || _u5_ufns=3D=3D1) { t=3D_u5_uGC(dc,"__utma=3D"+_u5_udh,";"); if ((i=3Dt.lastIndexOf(".")) > 9) { _u5_uns=3Dt.substring(i+1,t.length); _u5_uns=3D(_u5_uns*1); } cn++; if (_u5_uns=3D=3D0) _u5_uns=3D1; = _u5_ubd.cookie=3D"__utmz=3D"+_u5_udh+"."+_u5_ust+"."+_u5_uns+"."+cn+"."+c= +"; path=3D/; "+x+_u5_udo; } if (cs=3D=3D0 || _u5_ufns=3D=3D1) return "&utmcn=3D1"; else return "&utmcr=3D1"; } function _u5_uRef() { if (_u5_ur=3D=3D"0" || _u5_ur=3D=3D"" || _u5_ur=3D=3D"-") return ""; var i=3D0,h,k,n; if ((i=3D_u5_ur.indexOf("://"))<0) return ""; h=3D_u5_ur.substring(i+3,_u5_ur.length); if (h.indexOf("/") > -1) { k=3Dh.substring(h.indexOf("/"),h.length); if (k.indexOf("?") > -1) k=3Dk.substring(0,k.indexOf("?")); h=3Dh.substring(0,h.indexOf("/")); } h=3Dh.toLowerCase(); n=3Dh; if ((i=3Dn.indexOf(":")) > -1) n=3Dn.substring(0,i); for (var ii=3D0;ii<_u5_uRno.length;ii++) { if ((i=3Dn.indexOf(_u5_uRno[ii].toLowerCase())) > -1 && = n.length=3D=3D(i+_u5_uRno[ii].length)) { _u5_ufno=3D1; break; } } if (h.indexOf("www.")=3D=3D0) h=3Dh.substring(4,h.length); return = "utmccn=3D(referral)|utmcsr=3D"+_u5_uEC(h)+"|"+"utmcct=3D"+_u5_uEC(k)+"|u= tmcmd=3Dreferral"; } function _u5_uOrg(t) { if (_u5_ur=3D=3D"0" || _u5_ur=3D=3D"" || _u5_ur=3D=3D"-") return ""; var i=3D0,h,k; if ((i=3D_u5_ur.indexOf("://")) < 0) return ""; h=3D_u5_ur.substring(i+3,_u5_ur.length); if (h.indexOf("/") > -1) { h=3Dh.substring(0,h.indexOf("/")); } for (var ii=3D0;ii<_u5_uOsr.length;ii++) { if (h.indexOf(_u5_uOsr[ii]) > -1) { if ((i=3D_u5_ur.indexOf("?"+_u5_uOkw[ii]+"=3D")) > -1 || = (i=3D_u5_ur.indexOf("&"+_u5_uOkw[ii]+"=3D")) > -1) { k=3D_u5_ur.substring(i+_u5_uOkw[ii].length+2,_u5_ur.length); if ((i=3Dk.indexOf("&")) > -1) k=3Dk.substring(0,i); for (var yy=3D0;yy<_u5_uOno.length;yy++) { if (_u5_uOno[yy].toLowerCase()=3D=3Dk.toLowerCase()) { = _u5_ufno=3D1; break; } } if (t) return _u5_uEC(k); else return = "utmccn=3D(organic)|utmcsr=3D"+_u5_uEC(_u5_uOsr[ii])+"|"+"utmctr=3D"+_u5_= uEC(k)+"|utmcmd=3Dorganic"; } } } return ""; } function _u5_uBInfo(page) { var sr=3D"-",sc=3D"-",ul=3D"-",fl=3D"-",je=3D1; var n=3Dnavigator; if (self.screen) { sr=3Dscreen.width+"x"+screen.height; sc=3Dscreen.colorDepth+"-bit"; } else if (self.java) { var j=3Djava.awt.Toolkit.getDefaultToolkit(); var s=3Dj.getScreenSize(); sr=3Ds.width+"x"+s.height; } if (_u5_ujv=3D=3D"-" && (!page || page=3D=3D"")) { for (var i=3D5;i>=3D0;i--) { var t=3D""; _u5_ubd.write(t); if (_u5_ujv!=3D"-") break; } } if (n.language) { ul=3Dn.language.toLowerCase(); } else if (n.browserLanguage) { ul=3Dn.browserLanguage.toLowerCase(); } je=3Dn.javaEnabled()?1:0; if (_u5_uflash) fl=3D_u5_uFlash(); return = "&utmsr=3D"+sr+"&utmsc=3D"+sc+"&utmul=3D"+ul+"&utmje=3D"+je+"&utmjv=3D"+_= u5_ujv+"&utmfl=3D"+fl; } function _u5__utmSetTrans() { var e; if (_u5_ubd.getElementById) e=3D_u5_ubd.getElementById("utmtrans"); else if (_u5_ubd.utmform && _u5_ubd.utmform.utmtrans) = e=3D_u5_ubd.utmform.utmtrans; if (!e) return; var l=3De.value.split("UTM:"); var i,i2,c; if (_u5_userv=3D=3D0 || _u5_userv=3D=3D2) 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(window.location.hash.length > 0) window.history.back();=20 else window.location=3D"#"; } function goback() { window.history.back();=20 } // Movie embedding. 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getUpdate(typ,pr1,pr2,pid) { if (typ =3D=3D "state") { =09 // Play=20 if (pr1 =3D=3D 2) { =09 // Pause active movie if it is playing. if (currentItem !=3D null && currentItem !=3D pid) { thisMovie(currentItem).sendEvent('playpause'); } =09 // Save current movie currentItem =3D pid;=09 =09 // Flush active movie var on Stop (3) and Pause (0). } else if(pr1 =3D=3D 3 || pr1 =3D=3D 0) { = =20 currentItem =3D null; }=20 } }; // This is a javascript handler for the player and is always needed. function thisMovie(movieName) { if(navigator.appName.indexOf("Microsoft") !=3D -1) { return window[movieName]; } else { return document[movieName]; } }; // Replacing the jpeg with the movie var link =3D true; function playvideo(id,moviename,width,height) { if (document.getElementById) { var flvheight =3D height + 20; var so =3D new = SWFObject('/movies/mediaplayer.swf','js'+id,width,flvheight,'7'); =20 so.useExpressInstall('/movies/expressinstall.swf');=20 so.addParam('allowfullscreen','true'); so.addParam('salign','lt'); so.addParam('quality','high'); so.addParam('scale','noscale'); so.addVariable('file',moviename); =20 so.addVariable('autostart','true'); so.addVariable('showdigits','false'); so.addVariable('useaudio','false'); so.addVariable('usefullscreen','true'); so.addVariable('showicons','false'); so.addVariable('enablejs','true'); so.addVariable('javascriptid','js'+id); = =20 so.addVariable('width',width); so.addVariable('height',flvheight); so.write(id); link =3D false; } } /** * SWFObject v1.5: Flash Player detection and embed - = http://blog.deconcept.com/swfobject/ * * SWFObject is (c) 2007 Geoff Stearns and is released under the MIT = License: * http://www.opensource.org/licenses/mit-license.php * */ if(typeof deconcept=3D=3D"undefined"){var deconcept=3Dnew = Object();}if(typeof = deconcept.util=3D=3D"undefined"){deconcept.util=3Dnew = Object();}if(typeof = deconcept.SWFObjectUtil=3D=3D"undefined"){deconcept.SWFObjectUtil=3Dnew = Object();}deconcept.SWFObject=3Dfunction(_1,id,w,h,_5,c,_7,_8,_9,_a){if(!= document.getElementById){return;}this.DETECT_KEY=3D_a?_a:"detectflash";th= is.skipDetect=3Ddeconcept.util.getRequestParameter(this.DETECT_KEY);this.= params=3Dnew Object();this.variables=3Dnew = Object();this.attributes=3Dnew = Array();if(_1){this.setAttribute("swf",_1);}if(id){this.setAttribute("id"= ,id);}if(w){this.setAttribute("width",w);}if(h){this.setAttribute("height= ",h);}if(_5){this.setAttribute("version",new = deconcept.PlayerVersion(_5.toString().split(".")));}this.installedVer=3Dd= econcept.SWFObjectUtil.getPlayerVersion();if(!window.opera&&document.all&= &this.installedVer.major>7){deconcept.SWFObject.doPrepUnload=3Dtrue;}if(c= ){this.addParam("bgcolor",c);}var = q=3D_7?_7:"high";this.addParam("quality",q);this.setAttribute("useExpress= Install",false);this.setAttribute("doExpressInstall",false);var = _c=3D(_8)?_8:window.location;this.setAttribute("xiRedirectUrl",_c);this.s= etAttribute("redirectUrl","");if(_9){this.setAttribute("redirectUrl",_9);= }};deconcept.SWFObject.prototype=3D{useExpressInstall:function(_d){this.x= iSWFPath=3D!_d?"expressinstall.swf":_d;this.setAttribute("useExpressInsta= ll",true);},setAttribute:function(_e,_f){this.attributes[_e]=3D_f;},getAt= tribute:function(_10){return = this.attributes[_10];},addParam:function(_11,_12){this.params[_11]=3D_12;= },getParams:function(){return = this.params;},addVariable:function(_13,_14){this.variables[_13]=3D_14;},g= etVariable:function(_15){return = this.variables[_15];},getVariables:function(){return = this.variables;},getVariablePairs:function(){var _16=3Dnew Array();var = key;var _18=3Dthis.getVariables();for(key in = _18){_16[_16.length]=3Dkey+"=3D"+_18[key];}return = _16;},getSWFHTML:function(){var = _19=3D"";if(navigator.plugins&&navigator.mimeTypes&&navigator.mimeTypes.l= ength){if(this.getAttribute("doExpressInstall")){this.addVariable("MMplay= 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IsValid(this.getAttribute("version"))){var n=3D(typeof = _20=3D=3D"string")?document.getElementById(_20):_20;n.innerHTML=3Dthis.ge= tSWFHTML();return = true;}else{if(this.getAttribute("redirectUrl")!=3D""){document.location.r= eplace(this.getAttribute("redirectUrl"));}}return = false;}};deconcept.SWFObjectUtil.getPlayerVersion=3Dfunction(){var = _23=3Dnew = deconcept.PlayerVersion([0,0,0]);if(navigator.plugins&&navigator.mimeType= s.length){var x=3Dnavigator.plugins["Shockwave = Flash"];if(x&&x.description){_23=3Dnew = deconcept.PlayerVersion(x.description.replace(/([a-zA-Z]|\s)+/,"").replac= e(/(\s+r|\s+b[0-9]+)/,".").split("."));}}else{if(navigator.userAgent&&nav= igator.userAgent.indexOf("Windows CE")>=3D0){var axo=3D1;var = _26=3D3;while(axo){try{_26++;axo=3Dnew = ActiveXObject("ShockwaveFlash.ShockwaveFlash."+_26);_23=3Dnew = deconcept.PlayerVersion([_26,0,0]);}catch(e){axo=3Dnull;}}}else{try{var = axo=3Dnew = ActiveXObject("ShockwaveFlash.ShockwaveFlash.7");}catch(e){try{var = axo=3Dnew ActiveXObject("ShockwaveFlash.ShockwaveFlash.6");_23=3Dnew = deconcept.PlayerVersion([6,0,21]);axo.AllowScriptAccess=3D"always";}catch= (e){if(_23.major=3D=3D6){return _23;}}try{axo=3Dnew = ActiveXObject("ShockwaveFlash.ShockwaveFlash");}catch(e){}}if(axo!=3Dnull= ){_23=3Dnew deconcept.PlayerVersion(axo.GetVariable("$version").split(" = ")[1].split(","));}}}return = _23;};deconcept.PlayerVersion=3Dfunction(_29){this.major=3D_29[0]!=3Dnull= ?parseInt(_29[0]):0;this.minor=3D_29[1]!=3Dnull?parseInt(_29[1]):0;this.r= ev=3D_29[2]!=3Dnull?parseInt(_29[2]):0;};deconcept.PlayerVersion.prototyp= e.versionIsValid=3Dfunction(fv){if(this.majorfv.major){return = true;}if(this.minorfv.minor){return true;}if(this.rev=3D0;i--){_2f[i].style.display=3D"none";for(var x in = _2f[i]){if(typeof = _2f[i][x]=3D=3D"function"){_2f[i][x]=3Dfunction(){};}}}};if(deconcept.SWF= Object.doPrepUnload){if(!deconcept.unloadSet){deconcept.SWFObjectUtil.pre= 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