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window.pageYOffset+eventY-this.css.contentheight : =
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function writeMenu(obj,item)
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function openWin(pg)
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if (pg.indexOf('special') >=3D 0 )
{
var attributes =3D 'toolbar=3D0,location=3D0,directories=3D0,status=3D0, =
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0,top=3D0';
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{
var attributes =3D 'toolbar=3D0,location=3D0,directories=3D0,status=3D0, =
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sealWin=3Dwindow.open(pg ,'',attributes);=20
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function valfld(field)
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function chkvldemail(email_address)
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         if(Validate_String(user_name) =3D=3D=3D false || =
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			alert("Please enter valid email address.");
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		 return(true);
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function Validate_String(string, return_invalid_chars)
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                   invalid_chars +=3D ', ' + charx;
                }
              }
            }                    =20
           =20
         //If the string does not contain invalid characters, the =
function will return true.
         //If it does, it will either return false or a list of the =
invalid characters used
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parameter.
         if(return_invalid_chars =3D=3D true && invalid_chars !=3D '')
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           if(last_comma !=3D -1)
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invalid_chars.length);
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           return(invalid_chars);
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function trimstr(par)
{

while (par.charAt(0)=3D=3D" "){
par=3Dpar.replace(" ","")
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i=3Dpar.length-1
while(par.charAt(i)=3D=3D" "){
par=3Dpar.slice(0,i)
i=3Di-1
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return par
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// var xyz =3D createBar(
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// border_width,
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// scroll_speed,
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var w3c=3D(document.getElementById)?true:false;
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if (flag!=3D''){
	h=3D13;
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h=3D10
var t=3D'<div id=3D"_xpbar'+(++N)+'" style=3D"visibility:visible; =
position:relative; overflow:hidden; width:'+w+'px; height:'+h+'px; =
background-color:'+bgc+'; border-color:'+brdC+'; =
border-width:'+brdW+'px; border-style:solid; font-size:1px;">';
t+=3D'<span id=3D"blocks'+N+'" style=3D"left:-'+(h*2+1)+'px; =
position:absolute; font-size:1px">';
for(i=3D0;i<blocks;i++){
t+=3D'<span style=3D"background-color:'+blkC+'; left:-'+((h*i)+i)+'px; =
font-size:1px; position:absolute; width:'+h+'px; height:'+h+'px; '
t+=3D(ie)?'filter:alpha(opacity=3D'+(100-i*(100/blocks))+')':'-Moz-opacit=
y:'+((100-i*(100/blocks))/100);
t+=3D'"></span>';
}
t+=3D'</span></div>';
document.write(t);
var =
bA=3D(ie)?document.all['blocks'+N]:document.getElementById('blocks'+N);
bA.bar=3D(ie)?document.all['_xpbar'+N]:document.getElementById('_xpbar'+N=
);
bA.blocks=3Dblocks;
bA.N=3DN;
bA.w=3Dw;
bA.h=3Dh;
bA.speed=3Dspeed;
bA.ctr=3D0;
bA.count=3Dcount;
bA.action=3Daction;
bA.togglePause=3DtogglePause;
bA.showBar=3Dfunction(){
this.bar.style.visibility=3D"visible";
}
bA.hideBar=3Dfunction(){
this.bar.style.visibility=3D"hidden";
}
bA.tid=3DsetInterval('startBar('+N+')',speed);
return bA;
}}

function startBar(bn){
var =
t=3D(ie)?document.all['blocks'+bn]:document.getElementById('blocks'+bn);
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t.ctr++;
if(t.ctr>=3Dt.count){
eval(t.action);
t.ctr=3D0;
}}else t.style.left=3D(parseInt(t.style.left)+t.h+1)+'px';
}

function togglePause(){
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clearInterval(this.tid);
this.tid=3D0;
}}

function togglePause(){
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------=_NextPart_000_0000_01C82906.DEC51660
Content-Type: multipart/alternative;
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------=_NextPart_001_002D_01C82906.DECC4250
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Content-Transfer-Encoding: quoted-printable
Content-Location: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2006;volume=2;issue=1;spage=24;epage=27;aulast=Aggarwal

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" =
"http://www.w3c.org/TR/1999/REC-html401-19991224/loose.dtd">
<HTML><HEAD><TITLE>Brain tumor and role of &#946;-carotene, a- =
tocopherol, superoxide dismutase and glutathione peroxidase Aggarwal =
Sarita, Subberwal Manju, kumar Sushil, Sharma Meenakshi - J Can Res =
Ther</TITLE>
<META http-equiv=3DContent-Type content=3D"text/html; =
charset=3Dwindows-1252">
<META=20
content=3D"Journal of Cancer Research and Therapeutics, full-text, =
online, electronic, e-journal, free access, open access, online =
submission"=20
name=3Dkeywords>
<META=20
content=3D"Official publication of Association of Radiation Oncology of =
India (AROI)"=20
name=3Ddescription><!--DC META DATA START-->
<META=20
content=3D"Brain tumor and role of &#946;-carotene, a- tocopherol, =
superoxide dismutase and glutathione peroxidase"=20
name=3DDC.Title><LINK =
href=3D"http://purl.org/metadata/dublin_core_elements#title"=20
rel=3DSCHEMA.dc>
<META content=3DAggarwal name=3DDC.Creator><LINK=20
href=3D"http://purl.org/metadata/dublin_core_elements#creator" =
rel=3DSCHEMA.dc>
<META content=3Dmanubansiwal@yahoo.co.in name=3DDC.Creator.Address><LINK =

href=3D"http://purl.org/metadata/dublin_core_elements#creator" =
rel=3DSCHEMA.dc>
<META=20
content=3D"Superoxidase-dismutase, Glutathione peroxidase, =
&amp;#946;-carotene, a-tocopherol, Brain tumor"=20
name=3DDC.Subject>
<META=20
content=3D"The erythrocyte levels of the antioxidant enzymes SOD and =
GPx,<b>  </b> and serum levels of antioxidants vitamins &#946; -carotene =
and &#946; -tocopherol were<b>  </b> estimated in various types of brain =
tumors, and were compared with the levels in controls. Statistically =
significant (P<.001) diminished levels of<b>  </b>&#946; - carotene, =
&#946; -tocopherol, SOD and GPx, were observed in all the brain tumor =
patients as compared to controls. Malignant tumor also showed a relative =
decrease in antioxidant levels as compared to benign tumors. Comparison =
of histopathological sections of brain tumors also suggested a inverse =
relationship between antioxidant level and grades of malignancy. Marked =
decrease in antioxidant levels may have a role in genesis of =
considerable oxidative stress in brain tumors. Further more, the degree =
of decline in antioxidant levels may indicate severity of malignancy in =
brain tumors."=20
name=3DDC.Description><LINK=20
href=3D"http://purl.org/metadata/dublin_core_elements#description" =
rel=3DSCHEMA.dc>
<META content=3D"Medknow Publications" name=3DDC.Publisher><LINK=20
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href=3D"http://purl.org/metadata/dublin_core_elements#publisher" =
rel=3DSCHEMA.dc>
<META=20
content=3D"Aggarwal  Sarita, Subberwal  Manju, kumar  Sushil, Sharma  =
Meenakshi"=20
name=3DDC.Contributor.PersonalName><LINK=20
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rel=3DSCHEMA.dc>
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<META=20
content=3Dhttp://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal=20
name=3DDC.Identifier><LINK=20
href=3D"http://purl.org/metadata/dublin_core_elements#identifier" =
rel=3DSCHEMA.dc>
<META scheme=3DISSN content=3D0973-1482 name=3DDC.Identifier><LINK=20
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                <TD width=3D"100%" colSpan=3D3><FONT =
class=3DtocAT><B>ORIGINAL=20
                  ARTICLE</B></FONT></TD></TR>
              <TR>
                <TD width=3D"33%" height=3D5></TD>
                <TD width=3D"33%" height=3D5></TD>
                <TD width=3D"34%" height=3D5></TD></TR>
              <TR>
                <TD class=3Dother width=3D"100%" colSpan=3D3><B>Year =
</B>:=20
                  2006&nbsp; |&nbsp; <B>Volume</B> : 2&nbsp; |&nbsp;=20
                  <B>Issue</B> : 1&nbsp; |&nbsp; <B>Page</B> : =
24-27</TD></TR>
              <TR>
                <TD class=3Dother width=3D"100%"=20
            colSpan=3D3>&nbsp;</TD></TR></TBODY></TABLE>
            <P><FONT class=3DsTitle>Brain tumor and role of =
&#946;-carotene, a-=20
            tocopherol, superoxide dismutase and glutathione=20
            peroxidase</FONT><BR><BR><FONT =
class=3DarticleAuthor>Aggarwal=20
            Sarita<SUP>1</SUP>, Subberwal Manju<SUP>1</SUP>, kumar=20
            Sushil<SUP>2</SUP>, Sharma =
Meenakshi<SUP>3</SUP></FONT><BR><FONT=20
            class=3DAuthorAff><SUP>1</SUP>&nbsp;Department of =
Biochemistry,=20
            Maulana Azad Medical College, New Delhi,=20
            India<BR><SUP>2</SUP>&nbsp;Deptt. of neurosurgery,G. B. Pant =

            Hospital, New Delhi, India<BR><SUP>3</SUP>&nbsp;Department =
of=20
            biochemistry and biotechnology National Institute of =
communicable=20
            diseases, New Delhi, India<BR></FONT><BR></P>
            <P><FONT class=3DCorrsAdd><B>Correspondence =
Address</B>:<BR>Subberwal=20
            Manju <BR>146A/AG-1, Vikaspuri, New Delhi=20
            <BR>India<BR>manubansiwal@yahoo.co.in</FONT> </P>
            <TABLE cellSpacing=3D0 cellPadding=3D0 width=3D"100%" =
border=3D0>
              <TBODY>
              <TR>
                <TD class=3DinsideLine width=3D"100%"=20
            height=3D1></TD></TR></TBODY></TABLE>&nbsp;<BR>
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
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            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub width=3D"85%" =
height=3D15>&nbsp;&gt;<A=20
                  name=3DAbstract></A>&nbsp;Abstract</TD>
                <TD class=3Dinthis align=3Dright>&nbsp;</TD>
                <TD width=3D"15%" height=3D15></TD></TR></TBODY></TABLE>
            <P>The erythrocyte levels of the antioxidant enzymes SOD and =
GPx,<B>=20
            </B>and serum levels of antioxidants vitamins &#946; =
-carotene and &#946;=20
            -tocopherol were<B> </B>estimated in various types of brain =
tumors,=20
            and were compared with the levels in controls. Statistically =

            significant (P&lt;.001) diminished levels of<B> </B>&#946; - =
carotene, &#946;=20
            -tocopherol, SOD and GPx, were observed in all the brain =
tumor=20
            patients as compared to controls. Malignant tumor also =
showed a=20
            relative decrease in antioxidant levels as compared to =
benign=20
            tumors. Comparison of histopathological sections of brain =
tumors=20
            also suggested a inverse relationship between antioxidant =
level and=20
            grades of malignancy. Marked decrease in antioxidant levels =
may have=20
            a role in genesis of considerable oxidative stress in brain =
tumors.=20
            Further more, the degree of decline in antioxidant levels =
may=20
            indicate severity of malignancy in brain tumors.</P><BR>
            <P><B>Keywords:</B>&nbsp;Superoxidase-dismutase, Glutathione =

            peroxidase, &amp;#946;-carotene, a-tocopherol, Brain =
tumor</P><BR>
            <TABLE class=3Dsitethis width=3D"100%" border=3D0>
              <TBODY>
              <TR>
                <TD class=3Dother><B>How to cite this =
article:</B><BR>Aggarwal=20
                  S, Subberwal M, kumar S, Sharma M. Brain tumor and =
role of=20
                  &#946;-carotene, a- tocopherol, superoxide dismutase =
and=20
                  glutathione peroxidase. J Can Res Ther=20
              2006;2:24-7</TD></TR></TBODY></TABLE><BR>
            <TABLE class=3Dsitethis width=3D"100%" border=3D0>
              <TBODY>
              <TR>
                <TD class=3Dother><B>How to cite this =
URL:</B><BR>Aggarwal S,=20
                  Subberwal M, kumar S, Sharma M. Brain tumor and role =
of=20
                  &#946;-carotene, a- tocopherol, superoxide dismutase =
and=20
                  glutathione peroxidase. J Can Res Ther [serial online] =
2006=20
                  [cited&nbsp;2007 Nov 17];2:24-7. Available =
from:&nbsp;<A=20
                  =
href=3D"http://www.cancerjournal.net/text.asp?2006/2/1/24/19771">http://w=
ww.cancerjournal.net/text.asp?2006/2/1/24/19771</A></TD></TR></TBODY></TA=
BLE><BR><BR><BR>
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub><A=20
                  =
name=3DIntroduction></A>&nbsp;&gt;&nbsp;Introduction</TD>
                <TD class=3Dinthis align=3Dright>&nbsp;</TD>
                <TD align=3Dright width=3D"15%"><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
MG=20
                  alt=3DTop=20
                  =
src=3D"http://www.cancerjournal.net/images/arrow_top.gif"=20
                  border=3D0></A></TD></TR></TBODY></TABLE><BR><BR>The =
role of oxidative=20
            stress in the genesis of various types of cancers is well=20
            established. Several chemical, cell culture and animal =
studies also=20
            indicate that antioxidants may slow or even prevent the =
development=20
            of cancer.<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref1"=20
            name=3Dft1>[1]</A> Antioxidant enzymes and vitamins are =
important=20
            group of cellularconstituents responsible for maintaining a=20
            balancebetween oxidants and antioxidants within a cell. =
Among the=20
            intracellular antioxidant enzymes, Superoxide-dismutase =
(SOD)and=20
            Glutathione Peroxidase (GPx) are responsiblefor the removal =
of ROS=20
            such as superoxide free radicals andH2O2. On the other hand =
vitamin=20
            E, breaks free radical chain reactions as a result of their =
ability=20
            to transfer a phenolic hydrogen to a peroxy free radical of =
a=20
            peroxidized polyunsaturated fatty acid (PUFA). There by =
preventing=20
            peroxidation of PUFA contained in cellular and subcellular =
membrane=20
            phospholipids. Similarly &#946;-carotene acts as an =
antioxidant by the=20
            stabilization of organic peroxide free radicals with in its=20
            conjugated alkyl structure. Since &#946;-carotene is =
effective at low=20
            oxygen concentrations, it complements the antioxidant =
properties of=20
            vitamin E which is effective at high oxygen concentration. =
Brain is=20
            considered abnormallysensitive to oxidative damage as brain =
tissue=20
            has high rate of oxygen consumption, high lipid content and=20
            relatively low antioxidant defenses, compared to other =
tissues.<A=20
            class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref2"=20
            name=3Dft2>[2]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref3"=20
            name=3Dft3>[3]</A> Relatively few studies have been done to =
estimate=20
            antioxidant <U>enzymes</U> in various brain tumors. Though =
most of=20
            the studies have shown decreased levels of these enzymes and =

            vitamins in various malignancies,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref4"=20
            name=3Dft4>[4]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref5"=20
            name=3Dft5>[5]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref6"=20
            name=3Dft6>[6]</A> the results are contradictory.<A =
class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref7"=20
            name=3Dft7>[7]</A> Study done in Finland (1994) demonstrated =
that lung=20
            cancer rates of male smokers increased significantly with =
&#946;-carotene=20
            supplementation,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref8"=20
            name=3Dft8>[8]</A> yet another study done by Omen GS, =
Goodman G,=20
            Thomquist M, et al. (1994) indicates a possible increase in =
lung=20
            cancer associated with antioxidants supplementation,<A =
class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref9"=20
            name=3Dft9>[9]</A> Although the totality of research =
supports health=20
            benefits from antioxidants, more studies are required before =
we can=20
            include them in our management of cancers.<BR><BR>In order =
to=20
            investigate the oxidative damage which might be involved in =
the=20
            chemical pathology of brain tumors and correlate them to the =

            histological findings and malignancy of tumors, we have =
examined the=20
            activities of various intracellular and extracellular =
antioxidants=20
            in different types of brain tumors and compared them with =
controls.=20
            <BR><BR><BR>
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub><A=20
                  name=3D"Materials and =
methods"></A>&nbsp;&gt;&nbsp;Materials and=20
                  methods</TD>
                <TD class=3Dinthis align=3Dright>&nbsp;</TD>
                <TD align=3Dright width=3D"15%"><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
MG=20
                  alt=3DTop=20
                  =
src=3D"http://www.cancerjournal.net/images/arrow_top.gif"=20
                  =
border=3D0></A></TD></TR></TBODY></TABLE><BR><BR>Clinical profile:=20
            Thirty clinically diagnosed cases of intracranial neoplasms =
between=20
            age group of 8-70 years were selected for the study. We =
excluded=20
            patients with known clinical conditions associated with =
altered=20
            lipid peroxidation and antioxidant status such as diabetes =
mellitus,=20
            ischemic states, hypertension, renal failure, arthritis, =
liver=20
            disease and pancreatitis by clinical history and relevant =
lab=20
            investigation. The samples were taken preoperative and their =

            diagnosis at the time was based on clinical findings and =
relevant=20
            investigations. The diagnosis was confirmed and staging was =
done by=20
            histopathological findings after the tumors were removed. =
Thirty age=20
            and sex matched healthy controls belonging to the same=20
            socio-economic status were also included in the=20
            study.<BR><BR>Collection of samples<B> :</B> Blood was =
freshly=20
            withdrawn by vene-puncture and it was transferred to tubes=20
            containing heparin for the estimation of SOD and GPx levels =
in=20
            erythrocytes. Red blood cells were separated by =
centrifugation and=20
            the plasma was discarded along with the buffy coat =
containing mainly=20
            white blood cells. RBC's were washed with 10 volumes of cold =
saline=20
            {for GPx estimation RBC were suspended in 10 volumes of cold =
buffer=20
            (50 mM tris-HCl, pH =3D 7.5 containing 5 mM EDTA and 1 mM=20
            mercaptoethanol)}. Erythrocyte lysate was resuspended in 4 =
packed=20
            cell volumes of ice cold water, samples were stored at -70 =
C, if not=20
            assayed immediately.<BR><BR>Procedure: &#946; -carotene and =
&#945;-tocopherol=20
            assay was done by Bradlay &amp; Hornbeck and Henson &amp; =
Warwick=20
            methods respectively.<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref10" =

            name=3Dft10>[10]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref11" =

            name=3Dft11>[11]</A> For superoxide-dismutase estimation, =
BIOXYTECH=20
            SOD -525=99 kit method<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref12" =

            name=3Dft12>[12]</A> was used . The SOD activity in units / =
ml was=20
            divided by the hemoglobin of the given lysate to express the =

            activity as U/mg of hemoglobin. For estimation of =
glutathione=20
            peroxidase, GPx-340=99 assay<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref13" =

            name=3Dft13>[13]</A> was used, which is an indirect measure =
of the=20
            activity of cytosolic Glutathione Peroxidase (c-GPx). The =
activity=20
            in original sample was expressed as per ml and it was =
divided with=20
            the hemoglobin content of the lysate.<BR><BR><B>Statistical=20
            analysis: </B>Statistical analysis was carried out according =
to=20
            student's paired and unpaired 't' test and correlation was=20
            calculated by Pearson's correlation coefficient. =
<BR><BR><BR>
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub><A=20
                name=3DResults></A>&nbsp;&gt;&nbsp;Results</TD>
                <TD class=3Dinthis align=3Dright>&nbsp;</TD>
                <TD align=3Dright width=3D"15%"><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
MG=20
                  alt=3DTop=20
                  =
src=3D"http://www.cancerjournal.net/images/arrow_top.gif"=20
                  border=3D0></A></TD></TR></TBODY></TABLE><BR><BR>We =
selected thirty=20
            patients of different types of intracranial tumors for our =
study.=20
            The male patients constituted 80 % and the females 20 % of =
the total=20
            number of cases. The maximum number of cancer patients in =
the study=20
            was in the age group of 31-40 years, 20 % of the total cases =
were in=20
            this age group. The range of the age of patients in our =
study was=20
            8-70 years. We estimated the levels of SOD, GPx, &#946; =
-carotene and=20
            &#945;-tocopherol in intracranial tumor and compared them =
with controls.=20
            The levels of superoxide-dismutase showed a statistically=20
            significant decrease in patients with intracranial neoplasms =
<A=20
            class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_1.jpg"=20
            target=3D_blank>[Table - 1]</A>, as compared to controls =
(P&lt;.001).=20
            63 % of the total tumor patients had SOD values lower than =
the=20
            lowest value of controls, similarly the levels of =
Glutathione=20
            Peroxidase in brain tumors was less than that of controls, =
which was=20
            found to be statistically significant (P&lt;.001) <A =
class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_1.jpg"=20
            target=3D_blank>[Table - 1]</A>. 46 % of the brain tumor =
cases had GPx=20
            value less than the lowest value of controls. The levels of=20
            &#946;-carotene and &#945;-tocopherol were also =
significantly (P&lt;.001)=20
            lower in patients of brain tumor as compared to that of =
controls <A=20
            class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_1.jpg"=20
            target=3D_blank>[Table - 1]</A>. 53 % of the total tumor =
cases had=20
            &#946;-carotene value less than the lowest value of controls =
and 80 % of=20
            the total brain tumor cases had &#946; -tocopherol value =
lower than the=20
            lowest value of controls.<BR><BR>When we compared =
antioxidant levels=20
            in benign brain tumors, with controls, we found that the =
levels of=20
            these antioxidants in benign tumors were significantly lower =
than=20
            the controls <A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_2.jpg"=20
            target=3D_blank>[Table - 2]</A>. The &#946; -carotene level =
was lower in=20
            malignant brain tumor than benign tumors, and the difference =
was=20
            found to be statistically significant (P&lt;.050). Although =
not=20
            statistically significant, &#946; -tocopherol and =
glutathione peroxidase=20
            levels in malignant tumors were considerably less than the =
benign=20
            tumor. After histopathological grading <A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_3.jpg"=20
            target=3D_blank>[Table - 3]</A> we found that Brain tumors =
of higher=20
            grades of malignancy for example astrocytoma, =
oligodendroglioma,=20
            oligodendroglioma G-III, glioblastoma multiforme, =
medulloblastoma=20
            and metastatic adenocarcinoma all showed proportionate =
decrease=20
            levels of &#946; -carotene, &#946; -tocopherol, =
superoxide-dismutase and=20
            glutathione peroxidase, as the tumor became more malignant.=20
            <BR><BR><BR>
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub><A=20
                  name=3DDiscussion></A>&nbsp;&gt;&nbsp;Discussion</TD>
                <TD class=3Dinthis align=3Dright>&nbsp;</TD>
                <TD align=3Dright width=3D"15%"><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
MG=20
                  alt=3DTop=20
                  =
src=3D"http://www.cancerjournal.net/images/arrow_top.gif"=20
                  border=3D0></A></TD></TR></TBODY></TABLE><BR><BR>In =
the present study,=20
            we found that serum &#946; -carotene and &#946; -tocopherol =
levels were=20
            significantly reduced in brain tumor patients as compared to =

            controls, and the values were even less in malignant tumors, =
when=20
            compared to relatively benign tumors. Our result was in =
close=20
            conformity with similar case studies done by other =
authors.<A=20
            class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref14" =

            name=3Dft14>[14]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref15" =

            name=3Dft15>[15]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref16" =

            name=3Dft16>[16]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref17" =

            name=3Dft17>[17]</A> Few studies have been done to compare =
the levels=20
            of these antioxidants in various histological types of brain =
tumors.=20
            We compared the levels of &#946;-carotene and &#946; =
-tocopherol in different=20
            types of brain tumors, we found that &#946; -carotene and =
&#946; -tocopherol=20
            levels decreases with increasing grades of malignancy and =
the=20
            decrease was statistically significant for oligodendroglioma =
G-I-II,=20
            glioblastoma multiforme and medulloblastoma. The protective =
effect=20
            seen can be because of the antioxidant properties of these =
two lipid=20
            soluble vitamins as various authors have suggested,<A =
class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref19" =

            name=3Dft19>[19]</A>,<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref1"=20
            name=3Dft1>[1]</A> beside their antioxidant action, several=20
            carotenoids show enhancement of the immune response, =
inhibition of=20
            mutagenesis, reduction of induced nuclear damage, similarly =
Vitamin=20
            E also has many non-antioxidant functions in the body such =
as=20
            anticoagulant, anti-inflammatory action, effects on gene =
regulation=20
            and immune function.<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref20" =

            name=3Dft20>[20]</A> The decrease levels of these nutrient=20
            antioxidants in cancer cases may have predisposed the =
patients to=20
            the development of tumor. <BR><BR>We also compared levels of =
SOD and=20
            GPx enzymes in cases of intracranial tumors, and we confirm =
the=20
            results of other authors<A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ref4"=20
            name=3Dft4>[4]</A> that levels of these enzymes were =
significantly=20
            less in brain tumor cases than in controls. When we =
separated the=20
            cases on the basis of histopathological type of tumor both =
enzymes=20
            SOD and GPx, showed a notable decrease as the tumor became =
more=20
            malignant, another study has also shown a proportionate =
decrease of=20
            superoxide-dismutase with the increasing grades of =
malignancy in=20
            brain tumor.21 The low levels of antioxidants in brain tumor =

            patients could be as a result of this increased oxidative =
damage; or=20
            it could be that low values aggravated the free radical =
damage and=20
            increased the chance of developing cancer, indicating =
antioxidants=20
            role in prevention and role of oxidative injury in the =
causation of=20
            cancer.<BR><BR>No global answer can yet be given about the =
role of=20
            individual antioxidant in human tumorigenesis. According to =
our=20
            findings it seems likely that the ability of scavenging =
oxygen free=20
            radicals was impaired in brain tumor patients than controls, =
because=20
            of the lowered levels of antioxidants which predispose the =
patient=20
            to harmful effects of carcinogens. Before we know exactly =
how it=20
            happens, a lot of lacunae need to be filled by further=20
            research.<BR><BR>&nbsp;
            <TABLE class=3DleftNav cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
            border=3D0>
              <TBODY>
              <TR>
                <TD class=3DpageSub height=3D15><A=20
                  name=3DReference></A>&nbsp;&gt;&nbsp;References</TD>
                <TD class=3Dinthis align=3Dright height=3D15>&nbsp;</TD>
                <TD align=3Dright width=3D"15%" height=3D15><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
MG=20
                  alt=3DTop=20
                  =
src=3D"http://www.cancerjournal.net/images/arrow_top.gif"=20
                  border=3D0></A></TD></TR></TBODY></TABLE><BR>
            <TABLE class=3Dbody cellSpacing=3D0 cellPadding=3D0 =
width=3D"100%"=20
              border=3D0><TBODY>
              <TR>
                <TD vAlign=3Dtop width=3D"5%"><A class=3Dref=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft1"=20
                  name=3Dref1>1.</A></TD>
                <TD>Guyton KZ, Kensler TW. Free radical in medicine. =
Cheeseman=20
                  KH (editor) Churchill Livingstone: Longman UK; 1993. =
p.=20
                  481-93. &nbsp;&nbsp;<A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft1"><I=
MG=20
                  alt=3D"Back to cited text no. 1"=20
                  =
src=3D"http://www.cancerjournal.net/images/ref_top.gif"=20
                  border=3D0></A>&nbsp;&nbsp;&nbsp;&nbsp;</TD></TR>
              <TR>
                <TD vAlign=3Dtop width=3D"5%"><A class=3Dref=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft2"=20
                  name=3Dref2>2.</A></TD>
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db=3DPubMed&amp;list_uids=3D12663945&amp;dopt=3DAbstract"=20
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p;acode=3DBTER-91-3-203"=20
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                  =
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db=3DPubMed&amp;list_uids=3D12498996&amp;dopt=3DAbstract"=20
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft7"=20
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IMG=20
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IMG=20
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft16"=20
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft17"=20
                  name=3Dref17>17.</A></TD>
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IMG=20
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                  border=3D0></A>&nbsp;&nbsp;&nbsp;&nbsp;</TD></TR>
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft18"><=
IMG=20
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                  border=3D0></A>&nbsp;&nbsp;&nbsp;&nbsp;</TD></TR>
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft19"><=
IMG=20
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src=3D"http://www.cancerjournal.net/images/ref_top.gif"=20
                  border=3D0></A>&nbsp;&nbsp;&nbsp;&nbsp;</TD></TR>
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                <TD vAlign=3Dtop width=3D"5%"><A class=3Dref=20
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href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft20"=20
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2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#ft20"><=
IMG=20
                  alt=3D"Back to cited text no. 20"=20
                  =
src=3D"http://www.cancerjournal.net/images/ref_top.gif"=20
                  =
border=3D0></A>&nbsp;&nbsp;&nbsp;&nbsp;</TD></TR></TBODY></TABLE><BR><BR>=

            <P class=3DpageSub>&nbsp;&nbsp;&nbsp;&nbsp;<A=20
            name=3Databs></A><B>Tables</B></P><A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_1.jpg"=20
            target=3D_blank>[Table - 1]</A>, <A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_2.jpg"=20
            target=3D_blank>[Table - 2]</A>, <A class=3Dref=20
            =
href=3D"http://www.cancerjournal.net/viewimage.asp?img=3DJCanResTher_2006=
_2_1_24_19771_3.jpg"=20
            target=3D_blank>[Table - 3]</A>=20
            <TABLE cellSpacing=3D0 cellPadding=3D0 width=3D"100%" =
border=3D0>
              <TBODY>
              <TR>
                <TD width=3D"100%">
                  <P align=3Dcenter><BR>
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                  <BR>&nbsp;</P></TD></TR>
              <TR>
                <TD align=3Dright width=3D"100%"><A=20
                  =
href=3D"http://www.cancerjournal.net/article.asp?issn=3D0973-1482;year=3D=
2006;volume=3D2;issue=3D1;spage=3D24;epage=3D27;aulast=3DAggarwal#top"><I=
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 s+=3D"&utmr=3D"+_ur;=0A=
 s+=3D"&utmp=3D"+pg;=0A=
 if ((_userv=3D=3D0 || _userv=3D=3D2) && _uSP()) {=0A=
  var i=3Dnew Image(1,1);=0A=
  i.src=3D_ugifpath+"?"+"utmwv=3D"+_uwv+s;=0A=
  i.onload=3Dfunction() {_uVoid();}=0A=
 }=0A=
 if ((_userv=3D=3D1 || _userv=3D=3D2) && _uSP()) {=0A=
  var i2=3Dnew Image(1,1);=0A=
  =
i2.src=3D_ugifpath2+"?"+"utmwv=3D"+_uwv+s+"&utmac=3D"+_uacct+"&utmcc=3D"+=
_uGCS();=0A=
  i2.onload=3Dfunction() { _uVoid(); }=0A=
 }=0A=
 return;=0A=
}=0A=
function _uVoid() { return; }=0A=
function _uCInfo() {=0A=
 if (!_ucto || _ucto=3D=3D"") { _ucto=3D"15768000"; }=0A=
 if (!_uVG()) return;=0A=
 var =
c=3D"",t=3D"-",t2=3D"-",t3=3D"-",o=3D0,cs=3D0,cn=3D0,i=3D0,z=3D"-",s=3D""=
;=0A=
 if (_uanchor && _udlh && _udlh!=3D"") s=3D_udlh+"&";=0A=
 s+=3D_udl.search;=0A=
 var x=3Dnew Date(_udt.getTime()+(_ucto*1000));=0A=
 var dc=3D_ubd.cookie;=0A=
 x=3D" expires=3D"+x.toGMTString()+";";=0A=
 if (_ulink && !_ubl) {=0A=
  z=3D_uUES(_uGC(s,"__utmz=3D","&"));=0A=
  if (z!=3D"-" && z.indexOf(";")=3D=3D-1) { =
_ubd.cookie=3D"__utmz=3D"+z+"; path=3D"+_utcp+";"+x+_udo; return ""; }=0A=
 }=0A=
 z=3Ddc.indexOf("__utmz=3D"+_udh);=0A=
 if (z>-1) { z=3D_uGC(dc,"__utmz=3D"+_udh,";"); }=0A=
 else { z=3D"-"; }=0A=
 t=3D_uGC(s,_ucid+"=3D","&");=0A=
 t2=3D_uGC(s,_ucsr+"=3D","&");=0A=
 t3=3D_uGC(s,"gclid=3D","&");=0A=
 if ((t!=3D"-" && t!=3D"") || (t2!=3D"-" && t2!=3D"") || (t3!=3D"-" && =
t3!=3D"")) {=0A=
  if (t!=3D"-" && t!=3D"") c+=3D"utmcid=3D"+_uEC(t);=0A=
  if (t2!=3D"-" && t2!=3D"") { if (c !=3D "") c+=3D"|"; =
c+=3D"utmcsr=3D"+_uEC(t2); }=0A=
  if (t3!=3D"-" && t3!=3D"") { if (c !=3D "") c+=3D"|"; =
c+=3D"utmgclid=3D"+_uEC(t3); }=0A=
  t=3D_uGC(s,_uccn+"=3D","&");=0A=
  if (t!=3D"-" && t!=3D"") c+=3D"|utmccn=3D"+_uEC(t);=0A=
  else c+=3D"|utmccn=3D(not+set)";=0A=
  t=3D_uGC(s,_ucmd+"=3D","&");=0A=
  if (t!=3D"-" && t!=3D"") c+=3D"|utmcmd=3D"+_uEC(t);=0A=
  else  c+=3D"|utmcmd=3D(not+set)";=0A=
  t=3D_uGC(s,_uctr+"=3D","&");=0A=
  if (t!=3D"-" && t!=3D"") c+=3D"|utmctr=3D"+_uEC(t);=0A=
  else { t=3D_uOrg(1); if (t!=3D"-" && t!=3D"") =
c+=3D"|utmctr=3D"+_uEC(t); }=0A=
  t=3D_uGC(s,_ucct+"=3D","&");=0A=
  if (t!=3D"-" && t!=3D"") c+=3D"|utmcct=3D"+_uEC(t);=0A=
  t=3D_uGC(s,_ucno+"=3D","&");=0A=
  if (t=3D=3D"1") o=3D1;=0A=
  if (z!=3D"-" && o=3D=3D1) return "";=0A=
 }=0A=
 if (c=3D=3D"-" || c=3D=3D"") { c=3D_uOrg(); if (z!=3D"-" && =
_ufno=3D=3D1)  return ""; }=0A=
 if (c=3D=3D"-" || c=3D=3D"") { if (_ufns=3D=3D1)  c=3D_uRef(); if =
(z!=3D"-" && _ufno=3D=3D1)  return ""; }=0A=
 if (c=3D=3D"-" || c=3D=3D"") {=0A=
  if (z=3D=3D"-" && _ufns=3D=3D1) { =
c=3D"utmccn=3D(direct)|utmcsr=3D(direct)|utmcmd=3D(none)"; }=0A=
  if (c=3D=3D"-" || c=3D=3D"") return "";=0A=
 }=0A=
 if (z!=3D"-") {=0A=
  i=3Dz.indexOf(".");=0A=
  if (i>-1) i=3Dz.indexOf(".",i+1);=0A=
  if (i>-1) i=3Dz.indexOf(".",i+1);=0A=
  if (i>-1) i=3Dz.indexOf(".",i+1);=0A=
  t=3Dz.substring(i+1,z.length);=0A=
  if (t.toLowerCase()=3D=3Dc.toLowerCase()) cs=3D1;=0A=
  t=3Dz.substring(0,i);=0A=
  if ((i=3Dt.lastIndexOf(".")) > -1) {=0A=
   t=3Dt.substring(i+1,t.length);=0A=
   cn=3D(t*1);=0A=
  }=0A=
 }=0A=
 if (cs=3D=3D0 || _ufns=3D=3D1) {=0A=
  t=3D_uGC(dc,"__utma=3D"+_udh,";");=0A=
  if ((i=3Dt.lastIndexOf(".")) > 9) {=0A=
   _uns=3Dt.substring(i+1,t.length);=0A=
   _uns=3D(_uns*1);=0A=
  }=0A=
  cn++;=0A=
  if (_uns=3D=3D0) _uns=3D1;=0A=
  _ubd.cookie=3D"__utmz=3D"+_udh+"."+_ust+"."+_uns+"."+cn+"."+c+"; =
path=3D"+_utcp+"; "+x+_udo;=0A=
 }=0A=
 if (cs=3D=3D0 || _ufns=3D=3D1) return "&utmcn=3D1";=0A=
 else return "&utmcr=3D1";=0A=
}=0A=
function _uRef() {=0A=
 if (_ur=3D=3D"0" || _ur=3D=3D"" || _ur=3D=3D"-") return "";=0A=
 var i=3D0,h,k,n;=0A=
 if ((i=3D_ur.indexOf("://"))<0) return "";=0A=
 h=3D_ur.substring(i+3,_ur.length);=0A=
 if (h.indexOf("/") > -1) {=0A=
  k=3Dh.substring(h.indexOf("/"),h.length);=0A=
  if (k.indexOf("?") > -1) k=3Dk.substring(0,k.indexOf("?"));=0A=
  h=3Dh.substring(0,h.indexOf("/"));=0A=
 }=0A=
 h=3Dh.toLowerCase();=0A=
 n=3Dh;=0A=
 if ((i=3Dn.indexOf(":")) > -1) n=3Dn.substring(0,i);=0A=
 for (var ii=3D0;ii<_uRno.length;ii++) {=0A=
  if ((i=3Dn.indexOf(_uRno[ii].toLowerCase())) > -1 && =
n.length=3D=3D(i+_uRno[ii].length)) { _ufno=3D1; break; }=0A=
 }=0A=
 if (h.indexOf("www.")=3D=3D0) h=3Dh.substring(4,h.length);=0A=
 return =
"utmccn=3D(referral)|utmcsr=3D"+_uEC(h)+"|"+"utmcct=3D"+_uEC(k)+"|utmcmd=3D=
referral";=0A=
}=0A=
function _uOrg(t) {=0A=
 if (_ur=3D=3D"0" || _ur=3D=3D"" || _ur=3D=3D"-") return "";=0A=
 var i=3D0,h,k;=0A=
 if ((i=3D_ur.indexOf("://")) < 0) return "";=0A=
 h=3D_ur.substring(i+3,_ur.length);=0A=
 if (h.indexOf("/") > -1) {=0A=
  h=3Dh.substring(0,h.indexOf("/"));=0A=
 }=0A=
 for (var ii=3D0;ii<_uOsr.length;ii++) {=0A=
  if (h.toLowerCase().indexOf(_uOsr[ii].toLowerCase()) > -1) {=0A=
   if ((i=3D_ur.indexOf("?"+_uOkw[ii]+"=3D")) > -1 || =
(i=3D_ur.indexOf("&"+_uOkw[ii]+"=3D")) > -1) {=0A=
    k=3D_ur.substring(i+_uOkw[ii].length+2,_ur.length);=0A=
    if ((i=3Dk.indexOf("&")) > -1) k=3Dk.substring(0,i);=0A=
    for (var yy=3D0;yy<_uOno.length;yy++) {=0A=
     if (_uOno[yy].toLowerCase()=3D=3Dk.toLowerCase()) { _ufno=3D1; =
break; }=0A=
    }=0A=
    if (t) return _uEC(k);=0A=
    else return =
"utmccn=3D(organic)|utmcsr=3D"+_uEC(_uOsr[ii])+"|"+"utmctr=3D"+_uEC(k)+"|=
utmcmd=3Dorganic";=0A=
   }=0A=
  }=0A=
 }=0A=
 return "";=0A=
}=0A=
function _uBInfo() {=0A=
 var sr=3D"-",sc=3D"-",ul=3D"-",fl=3D"-",cs=3D"-",je=3D1;=0A=
 var n=3Dnavigator;=0A=
 if (self.screen) {=0A=
  sr=3Dscreen.width+"x"+screen.height;=0A=
  sc=3Dscreen.colorDepth+"-bit";=0A=
 } else if (self.java) {=0A=
  var j=3Djava.awt.Toolkit.getDefaultToolkit();=0A=
  var s=3Dj.getScreenSize();=0A=
  sr=3Ds.width+"x"+s.height;=0A=
 }=0A=
 if (n.language) { ul=3Dn.language.toLowerCase(); }=0A=
 else if (n.browserLanguage) { ul=3Dn.browserLanguage.toLowerCase(); }=0A=
 je=3Dn.javaEnabled()?1:0;=0A=
 if (_uflash) fl=3D_uFlash();=0A=
 if (_ubd.characterSet) cs=3D_uES(_ubd.characterSet);=0A=
 else if (_ubd.charset) cs=3D_uES(_ubd.charset);=0A=
 return =
"&utmcs=3D"+cs+"&utmsr=3D"+sr+"&utmsc=3D"+sc+"&utmul=3D"+ul+"&utmje=3D"+j=
e+"&utmfl=3D"+fl;=0A=
}=0A=
function __utmSetTrans() {=0A=
 var e;=0A=
 if (_ubd.getElementById) e=3D_ubd.getElementById("utmtrans");=0A=
 else if (_ubd.utmform && _ubd.utmform.utmtrans) =
e=3D_ubd.utmform.utmtrans;=0A=
 if (!e) return;=0A=
 var l=3De.value.split("UTM:");=0A=
 var i,i2,c;=0A=
 if (_userv=3D=3D0 || _userv=3D=3D2) i=3Dnew Array();=0A=
 if (_userv=3D=3D1 || _userv=3D=3D2) { i2=3Dnew Array(); c=3D_uGCS(); }=0A=
=0A=
 for (var ii=3D0;ii<l.length;ii++) {=0A=
  l[ii]=3D_uTrim(l[ii]);=0A=
  if (l[ii].charAt(0)!=3D'T' && l[ii].charAt(0)!=3D'I') continue;=0A=
  var r=3DMath.round(Math.random()*2147483647);=0A=
  if (!_utsp || _utsp=3D=3D"") _utsp=3D"|";=0A=
  var f=3Dl[ii].split(_utsp),s=3D"";=0A=
  if (f[0].charAt(0)=3D=3D'T') {=0A=
   s=3D"&utmt=3Dtran"+"&utmn=3D"+r;=0A=
   f[1]=3D_uTrim(f[1]); if(f[1]&&f[1]!=3D"") =
s+=3D"&utmtid=3D"+_uES(f[1]);=0A=
   f[2]=3D_uTrim(f[2]); if(f[2]&&f[2]!=3D"") =
s+=3D"&utmtst=3D"+_uES(f[2]);=0A=
   f[3]=3D_uTrim(f[3]); if(f[3]&&f[3]!=3D"") =
s+=3D"&utmtto=3D"+_uES(f[3]);=0A=
   f[4]=3D_uTrim(f[4]); if(f[4]&&f[4]!=3D"") =
s+=3D"&utmttx=3D"+_uES(f[4]);=0A=
   f[5]=3D_uTrim(f[5]); if(f[5]&&f[5]!=3D"") =
s+=3D"&utmtsp=3D"+_uES(f[5]);=0A=
   f[6]=3D_uTrim(f[6]); if(f[6]&&f[6]!=3D"") =
s+=3D"&utmtci=3D"+_uES(f[6]);=0A=
   f[7]=3D_uTrim(f[7]); if(f[7]&&f[7]!=3D"") =
s+=3D"&utmtrg=3D"+_uES(f[7]);=0A=
   f[8]=3D_uTrim(f[8]); if(f[8]&&f[8]!=3D"") =
s+=3D"&utmtco=3D"+_uES(f[8]);=0A=
  } else {=0A=
   s=3D"&utmt=3Ditem"+"&utmn=3D"+r;=0A=
   f[1]=3D_uTrim(f[1]); if(f[1]&&f[1]!=3D"") =
s+=3D"&utmtid=3D"+_uES(f[1]);=0A=
   f[2]=3D_uTrim(f[2]); if(f[2]&&f[2]!=3D"") =
s+=3D"&utmipc=3D"+_uES(f[2]);=0A=
   f[3]=3D_uTrim(f[3]); if(f[3]&&f[3]!=3D"") =
s+=3D"&utmipn=3D"+_uES(f[3]);=0A=
   f[4]=3D_uTrim(f[4]); if(f[4]&&f[4]!=3D"") =
s+=3D"&utmiva=3D"+_uES(f[4]);=0A=
   f[5]=3D_uTrim(f[5]); if(f[5]&&f[5]!=3D"") =
s+=3D"&utmipr=3D"+_uES(f[5]);=0A=
   f[6]=3D_uTrim(f[6]); if(f[6]&&f[6]!=3D"") =
s+=3D"&utmiqt=3D"+_uES(f[6]);=0A=
  }=0A=
  if ((_userv=3D=3D0 || _userv=3D=3D2) && _uSP()) {=0A=
   i[ii]=3Dnew Image(1,1);=0A=
   i[ii].src=3D_ugifpath+"?"+"utmwv=3D"+_uwv+s;=0A=
   i[ii].onload=3Dfunction() { _uVoid(); }=0A=
  }=0A=
  if ((_userv=3D=3D1 || _userv=3D=3D2) && _uSP()) {=0A=
   i2[ii]=3Dnew Image(1,1);=0A=
   =
i2[ii].src=3D_ugifpath2+"?"+"utmwv=3D"+_uwv+s+"&utmac=3D"+_uacct+"&utmcc=3D=
"+c;=0A=
   i2[ii].onload=3Dfunction() { _uVoid(); }=0A=
  }=0A=
 }=0A=
 return;=0A=
}=0A=
function _uFlash() {=0A=
 var f=3D"-",n=3Dnavigator;=0A=
 if (n.plugins && n.plugins.length) {=0A=
  for (var ii=3D0;ii<n.plugins.length;ii++) {=0A=
   if (n.plugins[ii].name.indexOf('Shockwave Flash')!=3D-1) {=0A=
    f=3Dn.plugins[ii].description.split('Shockwave Flash ')[1];=0A=
    break;=0A=
   }=0A=
  }=0A=
 } else if (window.ActiveXObject) {=0A=
  for (var ii=3D10;ii>=3D2;ii--) {=0A=
   try {=0A=
    var fl=3Deval("new =
ActiveXObject('ShockwaveFlash.ShockwaveFlash."+ii+"');");=0A=
    if (fl) { f=3Dii + '.0'; break; }=0A=
   }=0A=
   catch(e) {}=0A=
  }=0A=
 }=0A=
 return f;=0A=
}=0A=
function __utmLinker(l,h) {=0A=
 if (!_ulink) return;=0A=
 var p,k,a=3D"-",b=3D"-",c=3D"-",x=3D"-",z=3D"-",v=3D"-";=0A=
 var dc=3D_ubd.cookie;=0A=
 if (!l || l=3D=3D"") return;=0A=
 var iq =3D l.indexOf("?"); =0A=
 var ih =3D l.indexOf("#"); =0A=
 if (dc) {=0A=
  a=3D_uES(_uGC(dc,"__utma=3D"+_udh,";"));=0A=
  b=3D_uES(_uGC(dc,"__utmb=3D"+_udh,";"));=0A=
  c=3D_uES(_uGC(dc,"__utmc=3D"+_udh,";"));=0A=
  x=3D_uES(_uGC(dc,"__utmx=3D"+_udh,";"));=0A=
  z=3D_uES(_uGC(dc,"__utmz=3D"+_udh,";"));=0A=
  v=3D_uES(_uGC(dc,"__utmv=3D"+_udh,";"));=0A=
  k=3D(_uHash(a+b+c+x+z+v)*1)+(_udh*1);=0A=
  =
p=3D"__utma=3D"+a+"&__utmb=3D"+b+"&__utmc=3D"+c+"&__utmx=3D"+x+"&__utmz=3D=
"+z+"&__utmv=3D"+v+"&__utmk=3D"+k;=0A=
 }=0A=
 if (p) {=0A=
  if (h && ih>-1) return;=0A=
  if (h) { _udl.href=3Dl+"#"+p; }=0A=
  else {=0A=
   if (iq=3D=3D-1 && ih=3D=3D-1) _udl.href=3Dl+"?"+p;=0A=
   else if (ih=3D=3D-1) _udl.href=3Dl+"&"+p;=0A=
   else if (iq=3D=3D-1) =
_udl.href=3Dl.substring(0,ih-1)+"?"+p+l.substring(ih);=0A=
   else _udl.href=3Dl.substring(0,ih-1)+"&"+p+l.substring(ih);=0A=
  }=0A=
 } else { _udl.href=3Dl; }=0A=
}=0A=
function __utmLinkPost(f,h) {=0A=
 if (!_ulink) return;=0A=
 var p,k,a=3D"-",b=3D"-",c=3D"-",x=3D"-",z=3D"-",v=3D"-";=0A=
 var dc=3D_ubd.cookie;=0A=
 if (!f || !f.action) return;=0A=
 var iq =3D f.action.indexOf("?"); =0A=
 var ih =3D f.action.indexOf("#"); =0A=
 if (dc) {=0A=
  a=3D_uES(_uGC(dc,"__utma=3D"+_udh,";"));=0A=
  b=3D_uES(_uGC(dc,"__utmb=3D"+_udh,";"));=0A=
  c=3D_uES(_uGC(dc,"__utmc=3D"+_udh,";"));=0A=
  x=3D_uES(_uGC(dc,"__utmx=3D"+_udh,";"));=0A=
  z=3D_uES(_uGC(dc,"__utmz=3D"+_udh,";"));=0A=
  v=3D_uES(_uGC(dc,"__utmv=3D"+_udh,";"));=0A=
  k=3D(_uHash(a+b+c+x+z+v)*1)+(_udh*1);=0A=
  =
p=3D"__utma=3D"+a+"&__utmb=3D"+b+"&__utmc=3D"+c+"&__utmx=3D"+x+"&__utmz=3D=
"+z+"&__utmv=3D"+v+"&__utmk=3D"+k;=0A=
 }=0A=
 if (p) {=0A=
  if (h && ih>-1) return;=0A=
  if (h) { f.action+=3D"#"+p; }=0A=
  else {=0A=
   if (iq=3D=3D-1 && ih=3D=3D-1) f.action+=3D"?"+p;=0A=
   else if (ih=3D=3D-1) f.action+=3D"&"+p;=0A=
   else if (iq=3D=3D-1) =
f.action=3Df.action.substring(0,ih-1)+"?"+p+f.action.substring(ih);=0A=
   else =
f.action=3Df.action.substring(0,ih-1)+"&"+p+f.action.substring(ih);=0A=
  }=0A=
 }=0A=
 return;=0A=
}=0A=
function __utmSetVar(v) {=0A=
 if (!v || v=3D=3D"") return;=0A=
 if (!_udo || _udo =3D=3D "") {=0A=
  _udh=3D_uDomain();=0A=
  if (_udn && _udn!=3D"") { _udo=3D" domain=3D"+_udn+";"; }=0A=
 }=0A=
 if (!_uVG()) return;=0A=
 var r=3DMath.round(Math.random() * 2147483647);=0A=
 _ubd.cookie=3D"__utmv=3D"+_udh+"."+_uES(v)+"; path=3D"+_utcp+"; =
expires=3D"+_uNx()+";"+_udo;=0A=
 var s=3D"&utmt=3Dvar&utmn=3D"+r;=0A=
 if ((_userv=3D=3D0 || _userv=3D=3D2) && _uSP()) {=0A=
  var i=3Dnew Image(1,1);=0A=
  i.src=3D_ugifpath+"?"+"utmwv=3D"+_uwv+s;=0A=
  i.onload=3Dfunction() { _uVoid(); }=0A=
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 if ((_userv=3D=3D1 || _userv=3D=3D2) && _uSP()) {=0A=
  var i2=3Dnew Image(1,1);=0A=
  =
i2.src=3D_ugifpath2+"?"+"utmwv=3D"+_uwv+s+"&utmac=3D"+_uacct+"&utmcc=3D"+=
_uGCS();=0A=
  i2.onload=3Dfunction() { _uVoid(); }=0A=
 }=0A=
}=0A=
function _uGCS() {=0A=
 var t,c=3D"",dc=3D_ubd.cookie;=0A=
 if ((t=3D_uGC(dc,"__utma=3D"+_udh,";"))!=3D"-") =
c+=3D_uES("__utma=3D"+t+";+");=0A=
 if ((t=3D_uGC(dc,"__utmb=3D"+_udh,";"))!=3D"-") =
c+=3D_uES("__utmb=3D"+t+";+");=0A=
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